2017 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Confirmed Posters

Presentations

Tackling Maple Syrup Urine Disease (MSUD) with Engineered Microbes
Yves Millet, Ning LI, Vincent M. Isabella, Binh Ha, Katie Walsh, Kelly Li, Kip West, Mary Castillo, Dean Falb, Paul Miller
Experimental Design Considerations for Efficient and Specific Gene Knock-in Using CRISPR-Cas9 for HDR Utilizing Synthetic RNAs
John A. Schiel, Hidevaldo Machado, Maren Mayer, Eldon Chou, Megan Basila, Melissa L. Kelley, Anja van Brabant Smith
Overcoming the Membrane Barrier: Recruitment of ?-Glutamyl Transferase for Intracellular Release of Cargo Molecules
Tilmann Kuenzl, Sven Panke, Philippe Marlière, Piet Herdewijn, Puneet Srivastava, Magdalena Sroka
Engineering of Lactobacillus Reuteri As a Biotherapeutic Delivery System
Laura Ortiz-Vélez, Laura Schaefer, Abigail Shulman, Jeff Tabor, Robert Britton
Development of a Microbial Biosensor for Detection of Inflammation-Associated Microenvironments
Jeffrey J. Tabor, Walter Chazin, Jeffrey Galley, Robert Britton, Kristina Daeffler
Structural Basis for the Enantioselectivity of Est-Y29 Toward (S)-Ketoprofen
Changsuk Oh, Tri Duc Ngo, Kyeong Kyu Kim
Probiotic Design through Microbial Community Modelling Using Genome-Scale Metabolic Models
Parizad Babaei
Tuning the Yeast MAPK Pathway for Yeast-Based Biosensors
Sarah Stainbrook, Keith Tyo
Active Degradation Allows for Increased Information Transmission Rate in Genetic Control
Nicholas A. Rossi, Mary J. Dunlop
Automating the Design of Digital Microfluidic Devices for Synthetic Biology
Mirela Alistar, Patrick Baudisch
Enlarging the Synthetic Biology Toolbox for Pichia Pastoris: Golden Gate Cloning and CRISPR/Cas9
Thomas Gassler, Roland Prielhofer, Lina Heistinger, Richard Zahrl, Franz Zehetbauer, Matthias Steiger, Michael Sauer, Brigitte Gasser, Diethard Mattanovich
Development of a Xylose Utilizing Saccharomyces Cerevisiae As a Platform for Lignocellulosic Biofuels/Biochemical Production
Sun-Mi Lee, Phuong Hoang Nguyen Tran
Characterizing  Nopaline Synthase Promoter Variants  for Use in Plant Synthetic Biology
Kevin Morey, June I. Medford
Towards a Cell-Free System for the Discovery of Post-Translationally Modified Peptide Natural Products
Benjamin P. Mohr, Dale A. Pelletier, Mitchel J Doktycz, Patricia M. Blair
Genetic Tool Development for Nannochloropsis species
Anne Ruffing, Jordan McEwen, Lucas Strickland
Improving the Efficiency of Microbial Isobutanol Synthesis: Directed Evolution Reveals Improved Enzymes and Optimal Enzyme Ratios
Indro Ghosh, Robert Landick
Development and Substrate Specificity Screening of an In Vivo Biosensor for the Detection of Biomass Derived Aromatic Chemical Building Blocks
Leopoldo F. M. Machado, Neil Dixon
Characterizing the Role of 23S rRNA Peptidyltransferase Center Nucleotides in Translation
Anne d'Aquino, Michael C. Jewett, Tasfia Azim
Scaling up DNA Data Storage
Chris Takahashi, Siena Dumas Ang, Lee Organick, Yuan-Jyue Chen, Randolph Lopez, Sergey Yekhanin, Konstantin Makarychev, Miklos Z. Racz, Sharon Newman, Bichlien Nguyen, Hsing-Yeh Parker, Cyrus Rashtchian, Kendall Stewart, Gagan Gupta, Robert Carlson, John Mulligan, Douglas Carmean, Georg Seelig, Luis Ceze, Karin Strauss
The Genetic Incompatibility of Metallo-?-Lactamases: Synthetic Biology Lessons from Directed Evolution
Ray Socha, Nobu Tokuriki
RNA-Guided Microbial Genome Editing Using Group II Introns and CRISPR-Cas Systems
Zhigang Zhang, Caitlin H. McCann, Gregory D. Davis, Erik R. Eastlund
Synthetic Morphogenesis in Plant Roots
Jose R. Dinneny, Jennifer A.N. Brophy
A Comprehensive Characterization of the Nitroreductase Superfamily
David Morales, Janine Copp, Eyal Akiva, Patsy Babbitt, Nobu Tokuriki
Engineered Control Systems for Asymmetric Cell Division in Synthetic Bacteria
Sara Molinari, David L. Shis, Oleg Igoshin, Matthew R. Bennett
Putative DNA-Binding Proteins Affecting the Subcellular Organization of Bacillus Subtilis
Sarah E. Hartman
Deep Device Mining for Lignin-Transforming Enzymes Using Whole-Cell Biosensors
Steven J. Hallam, Joe C.H. Ho, Sandip V. Pawar, Vikramaditya G. Yadav
Pbnj Gibson Cloning for Fast Turn-Around Donor Constructs in Gene-Editing Applications
Intisar K. Turner, Christine Chen
The Application of miRNA in Bone Tissue Engineering
Sue Anne Chew, Marco A. Arriaga, Astrid Gutierrez
A Next-Generation Multi-Omics Approach to Dissect Cellular and Molecular Reprogramming
Kamal Mandal, Souvik Sen Sharma, Gitanjali Yadav, Subeer S. Majumdar
Adaptive Landscapes and Molecular Basis for the Functional Innovation of an Organophosphate Hydrolysing Enzyme
Nobu Tokuriki, Colin J. Jackson, Florian Baier, Gloria Yang, Erich Bornberg-Bauer, Elias Dohmen, Dave Anderson
Synthetic analysis of natural variants yields insights into the evolution and function of auxin signaling F-box proteins in Arabidopsis thaliana
R. Clay Wright, Mollye L. Zahler, Stacey R. Gerben, Jennifer Nemhauser
Towards the Automated Design of Bacterial Genomes for Tailored Applications
Howard Salis
Engineering Bacterial S-Layer Protein Arrays to Generate Hybrid Nanomaterials
Francesca Manea, Caroline Ajo-Franklin
Single-Molecule Analysis of Synthetic RNA Devices in Living Cells By Fluorescence Cross-Correlation Spectroscopy
Eric Hayden, Matthew Ferguson, Jessica Roberts, Nathan Redman
Engineering Pulsatile Communication in Bacterial Consortia
James Parkin, Victoria Hsiao, Richard M. Murray
Development of a Conjugation-Based Genome Editing Tool in Shewanella Oneidensis MR-1 Using CRISPR/Cas9 and Recombineering
Anna Domènech Corts, Ryan T. Gill, Jeffrey A. Gralnick
Extracting input-output relationship of mammalian gene circuits using transient transfections
Christoph Stelzer, Yaakov Benenson
Engineering the Mammalian Gut Microbiome in Situ
Carlotta Ronda, Sway Chen, Vitor Cabral, Harris H. Wang
Composition and Prediction of Multi-Input Promoters
David Zong, Matthew R. Bennett, William Ott, Chinmaya Gupta
A rational approach for bio-production of recombinant proteins in mammalian cell systems
Raffaele Altamura, Jiten Doshi, Yaakov Benenson
Opportunitis for Facility-Enabled Science at the Doe Joint Genome Institute (JGI)
Yasuo Yoshikuni
An E coli whole Genome Mutant Library Enables Rapid Selection for Improved Fitness Under Diverse Stresses
Alaksh Choudhury, Zhiwen Wang, Dr. Ryan T. Gill, Joel L. Kaar
Easyclone-Markerfree: A Vector Toolkit for Marker-Less Integration of Genes into Saccharomyces Cerevisiae Via CRISPR-Cas9
Mathew jessop Fabre, Tadas Jakociunas, Vratislav Stovicek, Zongjie Dai, Michael Krogh Jensen, Jay Keasling, Irina Borodina
Engineering Autoregulation in Enzymatic Degradation Based Systems for Robust Dynamics and Improved Host Capacity
Marios Tomazou, Guy-Bart Stan
Controlling Energy Flow in Bacteria Using Engineered Ligand-Responsive Protein Electron Carriers
Jonathan J. Silberg, Joshua T. Atkinson, Ian J. Campbell, George N. Bennett, Jacob Torres
Cell-Sized Mechanosensitive and Biosensing Compartment Programmed with DNA
Allen P. Liu, Vincent Noireaux, Jonathan Garamella, Sagardip Majumder, Ying-Ling Wang
Tuning the Dials of Metabolite Biosensors
Di Liu, Diego Oyarzún, Ahmad Mannan, Fuzhong Zhang
Vectoreditorjs: An Open-Source Web-Based Javascript Application for DNA Sequence Visualization, Annotation, and Editing
Sarah LaFrance, Leanne Springthorpe, Hector Plahar, Jacob Coble, Thomas Rich, Nathan J Hillson, Joshua Nixon
Synthetic Sugar Cassettes for the Efficient Production of Flavonol Glycosides in Escherichia coli
Prakash Parajuli, Ramesh Prasad Pandey, Jae Kyung Sohng
Engineered Protein Materials for Selective Binding of Strategic Metals
Marimikel Charrier, Caroline Ajo-Franklin, Sharon Borglin