2016 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Accepted Posters

Presentations

Engineering Novel Modular Biosensors to Confer Metabolite-Responsive Regulation of Transcription
Andrew K.D. Younger, Neil C. Dalvie, Joshua Leonard
Loading As a Design Parameter for Genetic Circuits
Nithin Kumar, Hsin-Ho Huang, Domitilla Del Vecchio
Algae Culture in a Photobioreactor NEEDS Specific Growth MEDIA, LIGHT Distribution and Operational Aspects of Chemical Engineering
PROF. Nitosh Kumar Brahma
Yeast ribosome display for high-throughput selection of cap-independent translation regulatory elements impacting both in vitro and in vivo protein expression
Michael C. Jewett
RNA-Programmed Gene Circuit Engineering Using Crispr-Nickases
Kylie Standage-Beier, Xiao Wang, Qi Zhang
Rewiring Cellular Input-Output to Engineer Cell-Based Therapies That Interface Robustly with Human Physiology
Rachel M. Dudek, Joshua Leonard, Taylor Dolberg
Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype Isoprenoid Biosynthesis
Michael C. Jewett, Quentin Dudley
Haemoperfusive Membrane Encapsulated Activated Charcoal Packed Column Artificial Kidney in Replacing Haemodialysis
PROF. Nitosh Kumar Brahma
Novel luminescent reporters from marine environmental bacteria for prokaryotic and eukaryotic systems
Anton Kan, Bernardo Pollak, Jim Haseloff
Transhydrogenase Promotes the Robustness and Evolvability of Metabolic Networks
Hsin-Hung Chou, Uwe Sauer, Christopher J. Marx
Defining the Anti-Shine-Dalgarno Sequence Interaction and Quantifying Its Functional Role in Regulating Translation Efficiency
Adam J Hockenberry, Adam R Pah, Michael C. Jewett, Luis A.N. Amaral
Analysis of Yeast Metabolism to Overproduce Isobutanol and n-Butanol
Mingyuan Tian, Trey Sato, Jennifer L. Reed
Plasmid GENE Trasfer Invitro into Auxotroph Pheno-Genotypes, F-, Escherichia coli K-12, Generate Hybrid Prototrophs to  Damage Clonal Selectivity
PROF. Nitosh Kumar Brahma
Engineering DNA polymerase delta to make its activity Ca2+ dependent
Namita Bhan
RNA- Programmed DNA Methylation
Glenna Meister, Tina Xiong, Nathaniel Kato, Winston Timp, Carl Novina, Marc Ostermeier
Modeling of Cell-Free Glycoprotein Production in Escherichia coli
Nicholas G. Horvath, Michael Vilkhovoy, Jeffery D. Varner, James Swartz, Kara A. Calhoun
ICE: A Distributed and Interconnected Biological Part Registry
Hector Plahar, Nathan J Hillson, Oge Nnadi, Joanna Chen, Timothy Ham, Zinovii Dmytriv, Paul D. Adams
Development of a Multi-Omics Approach to Study Differential Gene Regulation- a Potential Workflow for the Development and Validation of Synthetic Genetic Circuits
Kamal Mandal, Samuel L. Bader, Pankaj Kumar, Dipankar Malakar, David S. Campbell, Bhola Shankar Pradhan, Vaibhav Jain, Robert L. Moritz, Subeer S. Majumdar
Computationally Reconstructing RNA Folding Pathways with Co-Transcriptional SHAPE-Seq
Angela M. Yu, Eric J. Strobel
Understanding Cell Reprogramming By Adaptive Regulatory Mutations As a Guide for Cell Design
Jose Utrilla, Bernhard O. Palsson
Modulating Arginine Biosynthesis in E. coli nissle to Consume Ammonia in a Mouse Model of Hyperammonemia
Jonathan W. Kotula, Vincent M. Isabella, Dean Falb, Paul Miller, Alex Tucker, Alison Silva, Yves Millet, Cami Anderson
Discovering the Function of a Vibrio Fischeri Metabolic Gene Using a Newly Constructed Genome-Scale Model and Genomic Library
Shu Pan, Kiel Nikolakakis, Jennifer Reed, Edward Ruby
Designer Biosensors That Respond to New Small Molecules
Vatsan Raman
An RNA-Based Negative Autoregulation Circuit
Yan Zhang, Chelsea Hu
Conversion of Glucose to 2,3-Butanediol in Escherichia coli Cell-Free Lysates: A Model System
Michael C. Jewett
Unravelling RNA Structure / Function Relationships Via Novel Next-Generation Sequencing Techniques
Characterizing the Role of 23S rRNA Peptidyltransferase Center Interactions in Translation Fidelity
Anne d'Aquino, Evan Buechel, Yi Liu, Michael Jewett
Engineering a Growth Sensor to Detect Antigen-Antibody Interactions in Mammalian Cells
Thuy Duong Nguyen, Satoshi Inoue, Yoshihiro Kaku
Benchmarking RNA Switches for Diagnostic Applications
Matthew Verosloff
A Highly Productive One-Pot System for the Incorporation of Non-Standard Amino Acids into Cell-Free Synthesized Proteins
Michael C. Jewett
Using Volatile Gas Production As an Output for Genetic Circuits to Report on Programmed Microbial Behaviors in a Soil
Hsiao-Ying Cheng, Johnathan J. Silberg, Caroline A. Masiello
A Scalable Peptide/GPCR Communication Language
Michael Shen, Neta Agmon, Sonja Billerbeck, James Brisbois, Miguel Jimenez, Virginia Cornish, Jef Boeke
Random Homology-Aided Transformation for Genome and Plasmid Streamlining
Gabriel A. Suarez, Jeffrey E. Barrick
A Cell-Free Approach to Fine Chemical Pathway Engineering
Simon Moore, Karen Polizzi
One-Step in Vitro Large Gene Cluster Cloning By Crispr-Cas9 with Gibson Assembly
Chunbo Lou
Tuning the Electron Transfer Activity of a 2Fe2S Ferredoxin Using Homologous Recombination and an Escherichia coli Selection
Joshua T. Atkinson, Johnathan J. Silberg, Ian J. Campbell
Maintaining Plasmid Stability in Commensal Escherichia coli Nissle 1917 without Antibiotic Selection through Orthogonal Toxin-Antitoxin Systems
Tanel Ozdemir, Alex J H Fedorec, Chris Barnes
Synthetic Polyketide Enzymology: Platform for Biosynthesis of Antimicrobial Polyketides
Wen Shan Yew, Maybelle Kho Go, Jantana Wongsantichon, Vivian Wing Ngar Cheung, Jeng Yeong Chow, Robert C. Robinson
Orchestrating an Enzyme Ensemble Towards Novel Antibiotics
Hung-En Lai, Simon Moore, Karen Polizzi
Engineering Small Transcription Activating RNAs (STARs)
James Chappell
Strategies to Enhance the Yield of Biocellulose in Gluconacetobacter Xylinus
Prashant Kumar, Kevin McClay, Mark Fuller, Jennifer Reed
Recursive Cloning for the Assembly of Biosynthetic Pathways and Artificial CRISPR Arrays
Bob Van Hove, Lien De Wannemaeker, Chiara Guidi
Design Space Exploration of the Violacein Pathway in Escherichia coli Based Cell-Free System
Phuc Nguyen, Yong Wu, Shaobin Guo
In vitro glycosylation by cell-free protein synthesis-derived oligosaccharyltransferases
Jasmine M. Hershewe, Michael C. Jewett
Metabolic Engineering of Escherichia coli for Efficient Eukaryotic Glycan Biosynthesis
Cameron J. Glasscock
Deciphering the Regulation of Biomass Degradation By Anaerobic Fungi
John K. Henske, Michael K. Theodorou, Igor Grigoriev, Kevin V. Solomon
The Development, Implementation and Transfer of a Reproducible Methodology for the Generation of High Quality Data for Part Characterisation
Chueh Loo Poh, Richard I. Kitney, Christopher D. Hirst, Catherine M. Ainsworth
A General Strategy to Construct Small Molecule Biosensors in Eukaryotes 
Justin Feng
Single-Chain Variable Fragment Antibodies Targeting Amyloid β Oligomers: Alzheimer’s Disease Toxins
Erika Cline, Izolda Popova, Adriano Sebollela, Josette Kamel, Alex Qin, William Klein
Intracellular Spectral Recompositioning of Light: A Novel Strategy for Improving Photosynthetic Efficiency of Diatoms
Amphun Chaiboonchoe