2014 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Poster Session

Presentations

Engineering Transcriptional Regulator Effector Specificity through Rational Design and Rapid Prototyping
Emmanuel L.C. de los Santos, Joseph T. Meyerowitz, Stephen L. Mayo
Fatty Acid Responsive Hybrid Promoter Design in Oleaginous Yeast Yarrowia Lipolytica
An Orthogonal DNA Replication System in Yeast
Arjun Ravikumar, Chang Liu, Adrian Arrieta
Engineering Protein-Nucleic Acid Binding for Cellular Circuits
Ryan T. Gill, Anna Domènech Corts
Construction of a Metabolite Valve in Saccharomyces Cerevisiae Increases Pathway Yields
Sue Zanne Tan
Synthetic Approaches to Investigate Intracellular Transport and Cytoskeletal Regulatory Systems
Jan Zimak, Michael Diehl, David Tsao, Eric Kumar, Tyler McLaughlin
The Development of in Vitro-Based Biosensors for the Detection of Pathogenic Biofilms
Ke Yan Wen, James Chappell, Kirsten Jensen, Paul S. Freemont, Alain Filloux, Jane Davies
Quantitative Prediction of Therapeutic Fusion Protein Dynamics
Avi Robinson-Mosher, Jan-Hung Chen, Jeffrey Way, Pamela A. Silver
Quantitative Design of Layered Genetic Circuits
Alec Nielsen
Programmable Bacteria As Diagnostics of Gut Health
Jonathan Kotula
Synthetic Chromatin-Based Logic, Spatial Regulation, and Memory
Albert Keung
Controlled Measurements of Multiple Cellular Components in E. coli As a Resource for Integrative Computational Modeling of Cellular Subsystems
John Houser, Craig Barnhart, Daniel Boutz, Sean Carroll, Brittany Needham, Ophelia Papoulas, Viswanadham Sridhara, Dariya Sydykova, M. Stephen Trent, Jeffrey E. Barrick, Claus Wilke, Edward Marcotte
Mpath: Computationally-Aided Design of Synthetic Metabolic Pathways
Robert Sidney Cox
Improving Polyketide Production By Screening Heterologously Expressed Synthetic Propionyl-CoA Carboxylases
Gergana Vandova, Robert O'Brien, Colin Harvey, Maureen Hillenmeyer
Evolution of a Yeast Receptor for Non-Native Ligands Using a Novel High-Throughput Screening Method
Michael Reddick, Michael Sherer, Taylor Riley
Design and Implementation of a Biomolecular Concentration Tracker
Victoria Hsiao, Emmanuel L.C. de los Santos, Weston R. Whitaker
Mazf-Mediated Deletion System for Genome Engineering in Budding Yeast Saccharomyces Cerevisiae
Quanli Liu, Yuzhen Wu, Xiuming Zhang, Yanling Bai, Haijin Xu, Mingqiang Qiao
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription-Translation (TX-TL) Systems
Engineering Auto-Regulatory Genetic Circuits That Enable Programmable Biological Functionalities: Biotechnological Applications
Tat-Ming Lo, Matthew Wook Chang
Isocost Lines in the Cellular Economy: Gene Expression Is Coupled Due to Competition for Shared Resources
Andras Gyorgy, Jose I. Jimenez, Hattie Chung, Ron Weiss, Domitilla Del Vecchio
Functional Probiotics Engineered to Eradicate P. Aeruginosa
In Young Hwang, Matthew Wook Chang
Engineering of Small RNA Transcriptional Activators
James Chappell
Development and Characterization of Dynamic Controllers in Saccharomyces Cerevisiae
Wei Suong Teo, Matthew Wook Chang, Kai Sheng Hee
Targeting Chromatin: An Emerging Layer of Synthetic Control
James J Collins
Transporter Engineering for Improved Microbial Tolerance Against Biofuel Molecules
Binbin Chen, Matthew Wook Chang, Susanna Su Jan Leong, Jee Loon Foo
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free "Breadboard"
Dan Siegal-Gaskins, Zoltan A. Tuza, Jongmin Kim
Development of a Synthetic Biology Toolbox for Synechococcus Sp. PCC 7002
Anne Ruffing
Optimized in-Fusion Cloning System Demonstrates High Efficiency and Accuracy of Multi-Fragment Cloning
Steve Oh, Gia Jokhadze, Tommy Duong, Magnolia Bostick, Andrew Farmer
Creating Components for Synthetic Gene Networks
David Shis, Matthew Bennett, Faiza Hussain, Liskin Swint-Kruse, Sarah Meinhardt
A Low Cost, Customizable Turbidostat for Use in Synthetic Circuit Characterization
Chris Takahashi, Aaron Miller, Felix Ekness, Maitreya Dunham
Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and Crispr/Cas Toolkit in Human Cells
Samuel D. Perli, Lior Nissim, Alexandra Fridkin, Pablo Perez-Pinera, Timothy K. Lu
A Rapid, Label-Free, and Scalable Method for Characterizing Binding Properties of Small Molecule Aptamers
Maureen McKeague
RNA-Guided Nucleases As Programmable-Spectrum Antimicrobials and Microbial Population Sculptors
Mark Mimee, Robert Citorik, Timothy K. Lu
Recombinant Optimization of an Orthogonal Tryptophanyl Suppressor tRNA
Arti Pothukuchy, Randall Hughes
Reconstitution of Orphan Fungal Polyketide Gene Clusters in Saccharomyces Cerevisiae
James Li, Colin Harvey, Maureen Hillenmeyer
Engineering Autonomous Recombinase Switches
Olivier Borkowski, Pakpoom Subsoontorn, Drew Endy
Temporal Inactivation of DNA Repair Enables Highly Precise Genome Engineering in Escherichia coli
Akos Nyerges, Bálint Csörgo, György Pósfai, Csaba Pál
Crispr-Cas9 Assisted Recombineering in Lactobacillus Reuteri
Jan Peter van Pijkeren, Jeehwan Oh
Optimizing Multi-Gene Biological Systems Using High-Throughput DNA Assembly, Sequencing, and Model-Guided Search Strategies
Library of Azotobacter Vinelandii Identifies a Strain with Biofertilizer Potential
Lauren J. Eberhart, Brett M. Barney
Conditional siRNA Production in Human Cell Lysate Via Shape and Sequence Transduction with Small Conditional RNAs
Lisa M. Hochrein, Tianjia J. Ge, Maayan Schwarzkopf, Niles A. Pierce
Effective Dynamic Modeling of Transcriptional RNA Circuitry in Cell-Free Transcription-Translation (TX-TL) Systems
Chelsea Hu
Enhanced Isoprenoid Production through the Cofactor Metabolic Engineering in Saccharomyces Cerevisiae
Huiqing Chong
Rational Design for Enzyme Engineering Using a Next Generation Gene Synthesis Approach to Generate Combinatorial Variant Libraries
Ishtiaq Saaem, Michael Hudson, Li Kung, Daniel Schindler, Nicholas Guido, Devin Leake
Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast Ggamma Recruitment System
Nobuo Fukuda, Shinya Honda
Optogenetic Characterization Methods Overcome Key Challenges in Synthetic Biology
Jeff Tabor
A Modular Approach to the Design of Embedded Controllers for Chemical Reaction Networks
Carlo Cosentino, Mariaconcetta Bilotta, Francesco Montefusco, Rucha Sawlekar, Francesco Amato
Characterization of the Performance of Non-Fluorescent Genetic Constructs Via Indirect Competitive Construct Characterization (iCCC)
Ariel Hecht, Matthew S. Munson, Drew Endy, Marc Salit
Control Engineering Inspired Design Tools for Synthetic Biology
James Arpino, Edward Hancock, Ye Yuan, Guy-Bart Stan, Jorge Goncalves, Antonis Papachristodoulou, Mauricio Barahona, Marios Tomazou, Mariano Beguerisse
Harnessing Peroxisomes for Metabolic Engineering
Zachary N. Russ, William C. DeLoache