2014 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Poster Session

Presentations

Design and Implementation of a Biomolecular Concentration Tracker
Victoria Hsiao, Emmanuel L.C. de los Santos, Weston R. Whitaker
Crispr-Cas9 Assisted Recombineering in Lactobacillus Reuteri
Jan Peter van Pijkeren, Jeehwan Oh
Engineering Autonomous Recombinase Switches
Olivier Borkowski, Pakpoom Subsoontorn, Drew Endy
Recombinant Optimization of an Orthogonal Tryptophanyl Suppressor tRNA
Arti Pothukuchy, Randall Hughes
A Rapid, Label-Free, and Scalable Method for Characterizing Binding Properties of Small Molecule Aptamers
Maureen McKeague
A Low Cost, Customizable Turbidostat for Use in Synthetic Circuit Characterization
Chris Takahashi, Aaron Miller, Felix Ekness, Maitreya Dunham
Optimized in-Fusion Cloning System Demonstrates High Efficiency and Accuracy of Multi-Fragment Cloning
Steve Oh, Gia Jokhadze, Tommy Duong, Magnolia Bostick, Andrew Farmer
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free "Breadboard"
Dan Siegal-Gaskins, Zoltan A. Tuza, Jongmin Kim
Targeting Chromatin: An Emerging Layer of Synthetic Control
James J Collins
Engineering of Small RNA Transcriptional Activators
James Chappell
Isocost Lines in the Cellular Economy: Gene Expression Is Coupled Due to Competition for Shared Resources
Andras Gyorgy, Jose I. Jimenez, Hattie Chung, Ron Weiss, Domitilla Del Vecchio
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription-Translation (TX-TL) Systems
Library of Azotobacter Vinelandii Identifies a Strain with Biofertilizer Potential
Lauren J. Eberhart, Brett M. Barney
Improving Polyketide Production By Screening Heterologously Expressed Synthetic Propionyl-CoA Carboxylases
Gergana Vandova, Robert O'Brien, Colin Harvey, Maureen Hillenmeyer
Controlled Measurements of Multiple Cellular Components in E. coli As a Resource for Integrative Computational Modeling of Cellular Subsystems
John Houser, Craig Barnhart, Daniel Boutz, Sean Carroll, Brittany Needham, Ophelia Papoulas, Viswanadham Sridhara, Dariya Sydykova, M. Stephen Trent, Jeffrey E. Barrick, Claus Wilke, Edward Marcotte
Programmable Bacteria As Diagnostics of Gut Health
Jonathan Kotula
Quantitative Prediction of Therapeutic Fusion Protein Dynamics
Avi Robinson-Mosher, Jan-Hung Chen, Jeffrey Way, Pamela A. Silver
The Development of in Vitro-Based Biosensors for the Detection of Pathogenic Biofilms
Ke Yan Wen, James Chappell, Kirsten Jensen, Paul S. Freemont, Alain Filloux, Jane Davies
Synthetic Approaches to Investigate Intracellular Transport and Cytoskeletal Regulatory Systems
Jan Zimak, Michael Diehl, David Tsao, Eric Kumar, Tyler McLaughlin
Construction of a Metabolite Valve in Saccharomyces Cerevisiae Increases Pathway Yields
Sue Zanne Tan
Engineering Protein-Nucleic Acid Binding for Cellular Circuits
Ryan T. Gill, Anna Domènech Corts
An Orthogonal DNA Replication System in Yeast
Arjun Ravikumar, Chang Liu, Adrian Arrieta
Fatty Acid Responsive Hybrid Promoter Design in Oleaginous Yeast Yarrowia Lipolytica
Enhanced Gene Disruption at Specific Promoter Region By Simultaneous Digestion of ZFN or Crispr/Cas System
Wataru Nomura, Akemi Masuda, Hirokazu Tamamura
Marinobacter Aquaeolei VT8 As a Model for the Production of High-Value Neutral Lipids in Bacteria
Brett M. Barney
Modulation of gene expression via directed electrical signaling; voltage sensitive promoters
Yousef Okasheh, Nicholas M. Marshall, Ross McBee
Engineering Yeast as a Host for Modular Polyketide Production
Dmitriy Kolesnikov, Robert P. St Onge
Systematic comparison of enzymatic error correction methods using deep sequencing
Di Zhang, Nathan B. Lubock, George M. Church
Genome Wide Manipulation of Transcription by CRISPR-Cas9 Transcription Factors in Saccharomyces cerevisiae
Justin Smith, Ulrich Schlecht, Sundari Suresh, Ana Maria Aparicio, Lars M. Steinmetz, Ron W. Davis, Leopold Parts, Robert P. St Onge
Genetic Reporter Systems for Understanding the MAR operon in Escherichia Coli
Nicholas A. Rossi
Sensitive detection of proteasomal degradation using orthogonal gene circuits
Yimeng Zeng
An in silico Knowledge-Base for Crispr-Mediated Cell Engineering
Sridhar Ranganathan, Namritha Ravinder, Jon Chesnut, Kevin Clancy
Parasight V1.0 – a Synthetic Biology Approach for the Detection of Parasites
Alexander J. Webb, Richard Kelwick, Ciaran McKeown, Kirsten Jensen, Nicolas Kylilis, Geoff S. Baldwin, Tom Ellis, Paul S. Freemont
Near-Perfect Digital Switching in a Synthetic Biosensor Circuit Achieved through Temporal Control of Circuit's Genetic Makeup
Nicolas Lapique
Engineering Transcriptional Regulator Effector Specificity through Rational Design and Rapid Prototyping
Emmanuel L.C. de los Santos, Joseph T. Meyerowitz, Stephen L. Mayo
Highly Modular “Bow-Tie” Gene Circuits with Programmable Dynamic Behavior
Laura Prochazka, Bartolomeo Angelici, Benjamin Häfliger
Construction and Characterization of Synthetic Dual-Input Promoters
Ian Roney, Mads Kærn
Ultra-High-Throughput Screening of Enzyme Libraries with Droplet-Based Microfluidics
The Layered Analysis and Control of Biochemical Reaction Networks
Thomas P. Prescott, Antonis Papachristodoulou
High-Throughput Crispri Studies of Gene Function and Regulation in E. coli
Harneet S. Rishi, Esteban Toro, David Chen, Guillaume Cambray, Xiaowo Wang, Honglei Liu, Lei S. Qi, Adam P. Arkin
Harnessing Peroxisomes for Metabolic Engineering
Zachary N. Russ, William C. DeLoache
Characterization of the Performance of Non-Fluorescent Genetic Constructs Via Indirect Competitive Construct Characterization (iCCC)
Ariel Hecht, Matthew S. Munson, Drew Endy, Marc Salit
Optogenetic Characterization Methods Overcome Key Challenges in Synthetic Biology
Jeff Tabor
Rational Design for Enzyme Engineering Using a Next Generation Gene Synthesis Approach to Generate Combinatorial Variant Libraries
Ishtiaq Saaem, Michael Hudson, Li Kung, Daniel Schindler, Nicholas Guido, Devin Leake
Effective Dynamic Modeling of Transcriptional RNA Circuitry in Cell-Free Transcription-Translation (TX-TL) Systems
Chelsea Hu
Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and Crispr/Cas Toolkit in Human Cells
Samuel D. Perli, Lior Nissim, Alexandra Fridkin, Pablo Perez-Pinera, Timothy K. Lu
Controlled Gene Amplification Enables High, Stable, Selection Free Gene Expression in Saccharomyces Cerevisiae
Christina S. Nødvig, Line D. Buron, Tomas Strucko, Zofia Jarczynska, Louise Mølgård, Uffe H. Mortensen
Control Engineering Inspired Design Tools for Synthetic Biology
James Arpino, Edward Hancock, Ye Yuan, Guy-Bart Stan, Jorge Goncalves, Antonis Papachristodoulou, Mauricio Barahona, Marios Tomazou, Mariano Beguerisse
A Modular Approach to the Design of Embedded Controllers for Chemical Reaction Networks
Carlo Cosentino, Mariaconcetta Bilotta, Francesco Montefusco, Rucha Sawlekar, Francesco Amato
Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast Ggamma Recruitment System
Nobuo Fukuda, Shinya Honda