2021 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Poster Presenters - Accepted

Presentations

gRNA-Seqret: Genome Wide Guide RNA Design and Sequence Extraction Tool
Ian K. Blaby, Jan-Fang Cheng, Lisa Simirenko
Redesign of Metabolic Pathways for High Yield Production of ?-Carotene in Yarrowia Lipolytica
Xena Pappalardo, Giorgio Jansen, Matteo Nicolò Amaradio, Jole Costanza, Giuseppe Nicosia
Ultrasensitive Indicator Gas Reporting Enables Biosensing in Native Soil Conditions
Emily M. Fulk, Xiaodong Gao, Kelly R. Redeker, Caroline A. Masiello, Johnathan J. Silberg
A Single-Layer Artificial Neural Network Type Architecture with Molecular Engineered Bacteria for Complex Conventional Computing
Kathakali Sarkar, Deepro Bonnerjee, Rajkamal Srivastava, Sangram Bagh
Construction of a Modular Framework for Orthogonal and Predictable Gene Expression in Prokaryotes
Shaunak Kar, Andrew Ellington, Jimmy Gollihar, Chad Wang, Cody Mcleland
Synthetic Epigenetic Reader Engineering with an All-in-One Cell-Free Expression and Protein Interaction Microarray
James Harrison, Neda Rafat, Aniruddh Sarkar, Karmella Haynes
Biological Design and Engineering By and for machine Learning
Jean-Loup Faulon, Leon Faure, Thomas Duigou, Manish Kushwaha
Bio-Production of Poly-DOPA As a Functional Material and Electron Acceptor for Extracellular Electron Transport and Bioelectrochemical Systems
David Walker
Beyond Fluorescein: Use of Fluorescent Protein Calibrants for Direct and Absolute Quantification of Protein Production in Synthetic Biology
Eszter Csibra, Guy-Bart Stan
Directed Evolution of a Generalist Biosensor Accelerates Alkaloid Pathway Engineering
Simon d'Oelsnitz, Andrew Ellington, Kamyab Javanmardi, Ross Thyer, Hal Alper
Automation Assisted Anaerobic Phenotyping for Metabolic Engineering
Kaushikraj Venkatesan, Radhakrishnan Mahadevan, Alexander F Yakunin, Patrick Diep, Sai Akhil Golla, Naveen Venayak
Dynamic Metabolic Control Improves NADPH Flux and Xylitol Biosynthesis in Engineered E. coli
Shuai Li, Michael Lynch
Engineering Bacteroides Thetaiotaomicron to Secrete Heterologous Therapeutic Protein
Suyue Lyu
Rapid Characterization of Spike Variants Via Mammalian Cell Surface Display
Kamyab Javanmardi
Cell-Free Detection of Heavy Metals for Rapid, in-Field Water Quality Testing
Casey Bernhards, Kathryn Beabout, Meghna Thakur, Kendrick Turner, Jorge Chávez, Scott Walper, Matthew Lux
Lysate Proteome Engineering Enables High-Yield Metabolite Conversions in Crude Cell Extracts
David C. Garcia, Jaime Lorenzo N. Dinglasan, Him Shrestha, Paul E. Abraham, Robert L. Hettich, Mitchel J. Doktycz
Synthetic Promoter Design in Escherichia coli Based on a Deep Generative Network
Ye Wang, Haochen Wang, Lei Wei, Liyang Liu, Xiaowo Wang
Portable Bacterial CRISPR Transcriptional Activation Enables Metabolic Engineering in Multiple Bacterial Species
Cholpisit Kiattisewee, Jason Fontana, Chen Dong, Widianti Sugianto, Ava Karanjia, Pamela Peralta-Yahya, James Carothers, Jesse Zalatan
Optogenetic Control of Antibiotic Resistance to Understand Impact of Activation Dynamics on Bacterial Survival
Michael B. Sheets, Mary J. Dunlop
Characterizing microRNA-Mediated Modulation of Gene Expression Noise and Its Effect on Synthetic Gene Circuits
Lei Wei, Shuailin Li, Xiaowo Wang
Metabolic Burden of CRISPR-Based Genetic Control Suggests Viability of Large Circuits for Control of Metabolic Pathway Expression
Ian Faulkner, James Carothers
Multi-Modal Engineering of Bst DNA Polymerase for Thermostability in Ultra-Fast Lamp Reactions
Andrew D. Ellington, Inyup Paik, Sanchita Bhadra, Raghav Shroff, Andre C. Maranhao, Jaydin Ngo
Bioprospecting in Algal Genomes with Conserved Gene Proximity
Crysten Blaby-Haas, Sean McCorkle
The Tryptophan-Induced tnaC Ribosome Stalling Sequence Exposes High Amino Acid Cross-Talk That Can be Mitigated By Removal of NusB for Higher Orthogonality
Mark Sherman, Lydia Contreras, Sanjna Sandeep
Contact-Free Detection of Sars-Cov-2 Via a Cell-Free Circuit to Resonant Sensor Interface
Jared Dopp, Adam Carr, Kaiyue Wu, Peivand Sadat Mousavi, Yeong Ran Jo, Keith Pardee, Alexander A. Green, Nigel Reuel
Biocatalytic Synthesis of Human Milk Oligosaccharides Lacto-N-Biose Via Modular Enzymatic Cascade with ATP Regeneration
Zhiqiang Du, Zhengyao Liu, Yinshuang Tan, Xu Fang
Using CRISPR/cas9 Editing to Explore the Spontaneous Adaptation of Ion Selectivity in a Bacterial Flagellar Motor
Pietro Ridone, Tsubasa Ishida, Yoshiyuki Sowa, Matthew Baker
Recombinant Cell-Free Extracts for Biocatalytic Cascades: Fertilizers to Diagnostics
Apostolos Alissandratos
Engineering and Directed Evolution of Cellular Protein Translation
Ahmed Badran
Genetic Engineering Strategies to Improve Vaccine Effectiveness and Production
Mark E Woodcock, Timothy J Doran, Kristie A Jenkins, Mark LV Tizard, Andrew GD Bean
Phage-Assisted Evolution of Botulinum Neurotoxin Proteases with Reprogrammed Specificity
Travis Blum
Construction of Synthetic Human Antibody Library Based on Repertoire Sequencing
Hiroto Katoh, Shumpei Ishikawa, Daisuke Komura
Crage Enables Rapid Activation of Biosynthetic Gene Clusters in Undomesticated Bacteria
Yasuo Yoshikuni
Digital Genome Engineering: Unlocking the Unlimited Potential of Biology
Nandini Krishnamurthy, Eric Abbate, Aaron Brooks, Katherine Krouse, Bryan Leland, Jacqueline Rosains, Tyson Shepherd
Systematically Delivering Biodegradable Mesoporous Silicananoparticles Via Dissolvable Microneedles to Promote Remyelination in Patients with Neurodegenerative Disease
Archit Kalra, Raghav Sriram, Geetika Chitturi, Krish Jayarapu
Engineered Probiotics Enabling Specific Sensing of Neurochemicals
Tae Seok Moon, Austin Rottinghaus, Chenggang Xi, Matthew Amrofell, Hyojeong Yi
Strategies for Quantifying and Enhancing Genetic Code Manipulation in Yeast
James Van Deventer, Jessica T. Stieglitz, Kelly Potts, Matthew Zackin, Ming Lei
Developing a Novel Microbial Chassis for Upcycling Waste Polyethylene Terephthalate
Tae Seok Moon, Jinjin Diao, Yifeng Hu
Simple Tools for Rapid Measurements of Evolving Fitness Distributions
Abe Pressman, David Ross, Drew S. Tack
Deep Mutational Scanning (DMS) Guided Therapeutic Enzyme Engineering
Nikhil Nair
Reaping Sweet Benefits of Industrialized Synthetic Biology: A Case Study of Successful Manufacturing Scale-up for Rebaudioside M
Aditi Khankhoje, Gale Wichmann
Abiotic Fluorescent Probes for Rapid Screening of Membrane-Disrupting Antibiotics
Nathaniel Kallmyer, Nigel Reuel, Danielle Eeg, Rachel Khor, Nathan Roby
Engineering Protein Secretion Platforms for Living Materials in Caulobacter Crescentus
Maria T. Orozco Hidalgo
Precise, Single-Cell Optogenetic Induction of Antibiotic Resistance for Selection of Escherichia coli
Caroline M. Blassick, Mary J. Dunlop
FireProtASR: Fully Automated Pipeline for Resurrecting Stable and Expressible Ancestral Proteins
Rayyan T. Khan, Milos Musil, Jan Stourac, David Bednar, Jiri Damborsky
Marrying Protein Synthetic Biology and Bioelectronic Transistors for the Rapid Detection of Single Protein Molecules (SARS-CoV-2 or otherwise)
Raik Grünberg, Keying Guo, Shofarul Wustoni, Anil Koklu, Escarlet Diaz-Galicia, Maximilian Moser, Adel Hama, Adeel Nazir Ahmad, Iain McCulloch, Stefan Arold, Sahika Inal
In Vitro Fermentation Test Bed for Evaluation of Engineered Sense and Respond Probiotics in Polymicrobial Communities
Steven Arcidiacono, Laurel A. Doherty, Jordan Whitman, Ida Pantoja-Feliciano, Jason Soares, Michael S. Goodson, Amy M. Ehrenworth Breedon, Wanda Lyon, Grace Jimenez, Scott Walper, Joseph R. Spangler
Synthetic Biology Approaches for the Optimization and Improvement of a Live Bacterial Therapeutic for the Treatment of Phenylketonuria (PKU)
Catherine Monahan
Evolution-Guided Engineering of Elongation Factor-Tu for Incorporation of Noncanonical Amino Acids
Lanqiao Xiong, Liliam Martinez Bello, Mariko F. Matsuura, Vanessa E. DeLey Cox, Eric A. Gaucher
Time-Course Expression Profiling Reveals Early and Late Responders to a Synthetic Chromatin Regulator
Isioma Enwerem-Lackland, Natecia L. Williams, Christopher Plaisier, Karmella Haynes