2021 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Poster Presenters - Accepted

Presentations

A Synthetic Live Attenuated Bacterial Vaccine for Countering Autoimmune Disease
Paul de Figueiredo, Jugal Das, Robert Alaniz, Jianxun Song, Thomas Ficht, Fengguang Guo
A Synthetic Chromatin Reader-Effector Activates Key Silenced Tumor Suppressor Genes in Triple Negative Breast Cancer Cells
Natecia L. Williams, Cara Shields, Lauren Hong, Karmella Haynes
Automated Design of Genetically-Encoded Protein-Sensing Riboswitches in a Cell-Free Expression System
Grace E. Vezeau, Lipika R. Gadila, Howard Salis
Using Newly-Identified Epigenetic Reader-Effector BAHCC1 to Engineer a H3K27me3-Binding Transcriptional Activator
Paige Steppe, Karmella Haynes
A Targeted Genome-Wide Approach to Elucidate and Control Bacterial Adhesion to Physicochemically Diverse Biomaterials
Stephanie Call, Brandon Ugbesia, Vanessa Vu, Lauren B. Andrews
Enhanced Regulation of Prokaryotic Gene Expression By a Eukaryotic Transcriptional Activator
Jonathan Emmons
Towards Dynamic and Complex RNA Circuits: A Novel Approach to Address Retroactivity with Positive Feedback Insulation
Baiyang Liu, James Chappell
Ultrasensitive Indicator Gas Reporting Enables Biosensing in Native Soil Conditions
Emily M. Fulk, Xiaodong Gao, Kelly R. Redeker, Caroline A. Masiello, Johnathan J. Silberg
Synthetic Epigenetic Reader Engineering with an All-in-One Cell-Free Expression and Protein Interaction Microarray
James Harrison, Neda Rafat, Aniruddh Sarkar, Karmella Haynes
Beyond Fluorescein: Use of Fluorescent Protein Calibrants for Direct and Absolute Quantification of Protein Production in Synthetic Biology
Eszter Csibra, Guy-Bart Stan
Dynamic Metabolic Control Improves NADPH Flux and Xylitol Biosynthesis in Engineered E. coli
Shuai Li, Michael Lynch
Cell-Free Detection of Heavy Metals for Rapid, in-Field Water Quality Testing
Casey Bernhards, Kathryn Beabout, Meghna Thakur, Kendrick Turner, Jorge Chávez, Scott Walper, Matthew Lux
Portable Bacterial CRISPR Transcriptional Activation Enables Metabolic Engineering in Multiple Bacterial Species
Cholpisit Kiattisewee, Jason Fontana, Chen Dong, Widianti Sugianto, Ava Karanjia, Pamela Peralta-Yahya, James Carothers, Jesse Zalatan
Metabolic Burden of CRISPR-Based Genetic Control Suggests Viability of Large Circuits for Control of Metabolic Pathway Expression
Ian Faulkner, James Carothers
The Tryptophan-Induced tnaC Ribosome Stalling Sequence Exposes High Amino Acid Cross-Talk That Can be Mitigated By Removal of NusB for Higher Orthogonality
Mark Sherman, Lydia Contreras, Sanjna Sandeep
Using CRISPR/cas9 Editing to Explore the Spontaneous Adaptation of Ion Selectivity in a Bacterial Flagellar Motor
Pietro Ridone, Tsubasa Ishida, Yoshiyuki Sowa, Matthew Baker
Engineering and Directed Evolution of Cellular Protein Translation
Ahmed Badran
Phage-Assisted Evolution of Botulinum Neurotoxin Proteases with Reprogrammed Specificity
Travis Blum
Crage Enables Rapid Activation of Biosynthetic Gene Clusters in Undomesticated Bacteria
Yasuo Yoshikuni
Systematically Delivering Biodegradable Mesoporous Silicananoparticles Via Dissolvable Microneedles to Promote Remyelination in Patients with Neurodegenerative Disease
Archit Kalra, Raghav Sriram, Geetika Chitturi, Krish Jayarapu
Strategies for Quantifying and Enhancing Genetic Code Manipulation in Yeast
James Van Deventer, Jessica T. Stieglitz, Kelly Potts, Matthew Zackin, Ming Lei
Simple Tools for Rapid Measurements of Evolving Fitness Distributions
Abe Pressman, David Ross, Drew S. Tack
Reaping Sweet Benefits of Industrialized Synthetic Biology: A Case Study of Successful Manufacturing Scale-up for Rebaudioside M
Aditi Khankhoje, Gale Wichmann
Engineering Protein Secretion Platforms for Living Materials in Caulobacter Crescentus
Maria T. Orozco Hidalgo
FireProtASR: Fully Automated Pipeline for Resurrecting Stable and Expressible Ancestral Proteins
Rayyan T. Khan, Milos Musil, Jan Stourac, David Bednar, Jiri Damborsky
In Vitro Fermentation Test Bed for Evaluation of Engineered Sense and Respond Probiotics in Polymicrobial Communities
Steven Arcidiacono, Laurel A. Doherty, Jordan Whitman, Ida Pantoja-Feliciano, Jason Soares, Michael S. Goodson, Amy M. Ehrenworth Breedon, Wanda Lyon, Grace Jimenez, Scott Walper, Joseph R. Spangler
Evolution-Guided Engineering of Elongation Factor-Tu for Incorporation of Noncanonical Amino Acids
Lanqiao Xiong, Liliam Martinez Bello, Mariko F. Matsuura, Vanessa E. DeLey Cox, Eric A. Gaucher
Development of a Toggle Switch Based on Post-Transcriptional Regulation That Resists Epigenetic Silencing
Noreen Wauford, Breanna DiAndreth, Ron Weiss
Modularised Engineering of Regulatory Circuits to Expand Artificial Control Mechanisms for Metabolic Engineering in Yeast
Bingyin Peng, Naga C. Bandari, Zeyu Lu, Christopher Howard, Colin Scott, Matt Trau, Geoff Dumsday, Claudia E. Vickers
Harnessing the Central Dogma for Stringent Multi-Level Control of Gene Expression
F. Veronica Greco
Screening for Microbial Therapeutic Interactions in Malignancy
Deirdre Ricaurte, Yiming Huang, Harris Wang
Designing Synbio Products with the End in Mind – Techno-Economic Analyses (TEAs) As a Tool for Strategic Development
Anna M. Crumbley
Engineering Synthetic Gene Circuits for Bacterial Therapeutic Delivery In Vivo
Tetsuhiro Harimoto, Tiffany Chien, Jaeseung Hahn, Tal Danino
Engineering a Protein Biomaterial: 2D and 3D Cell Culture Applications of Caf1
Daniel T. Peters, Helen Waller, Adam Creigh, Gema Dura, Pawan Gulati, Mark A. Birch, Nick J. Reynolds, Zofia M. Chrzanowska-Lightowlers, Robert N. Lightowlers, David A. Fulton, Jeremy H. Lakey
Construction of a Modular Framework for Orthogonal and Predictable Gene Expression in Prokaryotes
Shaunak Kar, Andrew Ellington, Jimmy Gollihar, Chad Wang, Cody Mcleland
Temperature-Responsive Optogenetic Probes of Cell Signaling
Lukasz Bugaj, William Benman, Erin Berlew, Hao Deng, Caitlyn Parker, Ivan Kuznetsov, Arndt Siekmann, Brian Chow, Bomyi Lim
Cas9-Mediated Random Access in DNA Data Storage
Nicolas Cardozo, Karen Zhang, Jeff Nivala, Luis Ceze, Karin Strauss, Delaney Wilde, Charlie Anderson
A GPCR-Based Yeast Biosensor for Biomedical, Biotechnological and Point-of-Use Cannabinoid Determination
Karel Miettinen, Sotirios C. Kampranis, Nattawat Leelahakorn, Aldo Almeida, Lukas R. Hansen, Yong Zhao, Soren Bak, Dan Staerk
Optimizing Nanoporeters for Multiplexable Mammalian Reporting Systems
Aerilynn Nguyen, Delaney Wilde, Jeff Nivala
Using Metabolic Network Modeling to Design an Escherichia coli strain with Enhanced Outer Membrane Vesicle Production
Héctor Alejandro Ruiz-Moreno, Juan Daniel Valderrama-Rincon, Andrés F. González
Expanding Nature’s Catalytic Toolbox with Artificial Metalloenzymes
Tobias Vornholt, Markus Jeschek, Sven Panke, Thomas R. Ward
Automated Metabolic Rewiring Design for Optimized Succinate Production in Saccharomyces Cerevisiae
Giuseppe Nicosia, Jole Costanza, Giorgio Jansen, Matteo Nicolò Amaradio, Xena Pappalardo
Redesign of Metabolic Pathways for High Yield Production of ?-Carotene in Yarrowia Lipolytica
Xena Pappalardo, Giorgio Jansen, Matteo Nicolò Amaradio, Jole Costanza, Giuseppe Nicosia
Synthetic Promoter Design in Escherichia coli Based on a Deep Generative Network
Ye Wang, Haochen Wang, Lei Wei, Liyang Liu, Xiaowo Wang
Biocatalytic Synthesis of Human Milk Oligosaccharides Lacto-N-Biose Via Modular Enzymatic Cascade with ATP Regeneration
Zhiqiang Du, Zhengyao Liu, Yinshuang Tan, Xu Fang
Bioprospecting in Algal Genomes with Conserved Gene Proximity
Crysten Blaby-Haas, Sean McCorkle
Characterizing microRNA-Mediated Modulation of Gene Expression Noise and Its Effect on Synthetic Gene Circuits
Lei Wei, Shuailin Li, Xiaowo Wang
Automation Assisted Anaerobic Phenotyping for Metabolic Engineering
Kaushikraj Venkatesan, Radhakrishnan Mahadevan, Alexander F Yakunin, Patrick Diep, Sai Akhil Golla, Naveen Venayak
Bio-Production of Poly-DOPA As a Functional Material and Electron Acceptor for Extracellular Electron Transport and Bioelectrochemical Systems
David Walker
Macroscopic De-Novo Engineered Living Materials
Sara Molinari, Robert Tesoriero, Caroline Ajo-Franklin, Jayashree Soman