C1 Biorefinery By Designer Enzyme Complexes As Protein Synthesis Machinery on the Cell Surface of Ralstonia Eutropha

The designer microbes are a key biological technology that can be used for biorefinery. The use of complexed enzyme systems is one of the strategies for effective bioresources utilization and offers considerably greater potential through a highly ordered structural organization that enables enzyme proximity synergy. Carbon monoxide (CO) was successfully efficiently converted by functional complexes containing carbon monoxide dehydrogenase and carbon monoxide sensing heme protein with enhanced CO binding affinity. Carbon monoxide sensing heme protein acts as mediator for increasing of carbon monoxide dehydrogenase activity by substrate proximity and assembled complexes caused an efficiently conversion of CO gas with aid of Carbon monoxide sensing heme protein. An enzyme complex for biological conversion of CO to CO₂ was anchored on the cell surface of CO₂-utilizing Ralstonia eutrophaand successfully showed 3.3-fold increased conversion efficiency. These results suggest that the complexed system may be a promising strategy for C1 biorefinery as biological tools for production of biochemicals such as bioplastics. The design of enzyme and microbe is a useful strategy as tools in whole-cell biocatalyst systems for C1 biorefinery and has drawn considerable attention as an attractive strategy for bioprocess applications.