Invited Talk: The Use of Novel Fractionation and Isolation Technologies for the Recovery of Biomolecules with Application in Food Formulations

In accordance with the current trends in bioprocessing each day new extraction, recovery and purification techniques are design and applied for the isolation of biomolecules with different applications. In this regard, many of those biomolecules have application in the food industry as antimicrobials, antioxidants, structuring proteins, pigments, etc. Therefore, the application of these novel extraction, recovery and purification technique have a significant role in the development of new food products and food processes. One of the techniques with more potential for the extraction, primary recovery and partial purification are the Aqueous Two-Phase Systems (ATPS). These liquid-liquid fractionation systems have been characterized for the downstream processing of low molecular weight compounds, biomacromolecules, bioparticles and even whole cells. ATPS allow to achieve process integration and intensification, while presenting a biocompatible environment for most bioactives. To exemplify the potential of ATPS the development of a pilot-plant scale process for the recovery of B-Phycoerythrin (BPE) from Porphyridium cruentum is presented as example.