Enzymes, as highly efficient biocatalysts, play critical roles in biosensing, metabolic regulation, and disease therapy. However, their practical applications are hindered by poor stability and susceptibility to inactivation. Metal-organic frameworks (MOFs), with their tunable pore sizes, high surface areas, and biocompatibility, have emerged as ideal carriers for enzyme encapsulation. In this study, a novel MOF material was designed by optimizing ligand spatial arrangement and metal node distribution, significantly enhancing enzyme loading capacity and catalytic performance. Experimental results demonstrated that the encapsulation efficiency of this MOF for multiple enzymes (e.g., glucose oxidase, β-galactosidase, tyrosinase) improved by over 30% compared to ZIF-8. In biomedical applications, the MOF-based carrier successfully enabled intracellular metabolite detection (e.g., real-time glucose monitoring) and restored functional abnormalities in lysosome-deficient cell models through enzyme replacement therapy, offering a novel therapeutic strategy for inherited enzyme-deficiency diseases. This study not only expands the application boundaries of MOFs in biocatalysis but also provides innovative insights into the development of enzyme delivery systems for precision medicine.
