2015 Synthetic Biology: Engineering, Evolution & Design (SEED)

Session: Poster Session A

Presentations

05:30 PM

De Novo Designed Toehold Repressors
Paul D. Carlson
Unleashing the 21st Amino Acid: Removing the Sequence Constraints of Selenocysteine Incorporation
Ross Thyer, Scott A. Robotham, Jennifer S. Brodbelt, Andrew D. Ellington
The Directed Evolution of Halogenases for Late-Stage Functionalization of Bioactive Molecules
James Payne, Mary Andorfer, Landon Durak, Kyle Kunze, Catherine Poor, Duo-Sheng Wang, Jared Lewis
Modified Transposons for Enhancing Protein Function
Dana Nadler, Stacy-Anne Morgan, Kaitlyn Kortright, Benjamin Oakes, Avi Flamholz, David Savage
Real-Time Optogenetic Control of Intracellular Protein Concentration in Microbial Cell Cultures
Megan McClean, Justin Melendez, Benjamin Oakes, Michael Patel, Marcus Noyes
Engineering Auto-Regulatory Genetic Circuits That Enable Modular and Programmable Functionalities for Biotechnological Applications
Tat Ming Lo, Matthew Wook Chang
New Evidence for the Effect of Human Chromatin on Cas9-Mediated Editing
Rene Davis
Self-Assembly through Toehold-Mediated Four-Way Branch Migration
Jocelyn Kishi
Construction and Characterization of a Refactored Actinorhodin Biosynthetic Cluster
Sangeeta Nath, Cindi Hoover, Katherine Louie, Benjamin P. Bowen, Jeffrey Kim, Trent Northen, Edward Rubin, Nathan J Hillson, Samuel Deutsch, Jay D. Keasling
ICE: A Distributed and Interconnected Biological Part Registry
Hector Plahar, Joanna Chen, Nathan J Hillson, Timothy Ham, Jay D. Keasling, Zinovii Dmytriv
Automated Statistical Design of Experiments for Metabolic Engineering
Brian Olson, Paramvir Dehal
Quantitative Metabolic Modeling for Biofuel Production at the Joint Bioenergy Institute
Hector Garcia Martin, Garrett Birkel, Amit Ghosh, William Morrell, Nathanial Echols, Nathan J Hillson, Paul D. Adams, Jay D. Keasling
Probabilistic Design of RNA Based Genetic Components: Next Generation Rgr Devices
William Voje, Miles W. Gander
Metabolic Engineering for the Production of Medium-Chain Fatty Acids and Derivatives in Baker's Yeast
Hua Ling, Ruirui Liu, Kaisheng Hee, Matthew Wook Chang
Synthetic DNA Delay Circuits for Temporal Coordination
Dominic Scalise, Dylan Howie
Optogenetic Frequency Analysis of Bacterial Two-Component Systems and Synthetic Gene Circuits
Lucas A. Hartsough, Ken Groszman, Jeffrey J. Tabor
An optogenetic system for nuclear translocation of viral nanoparticles and tunable gene delivery
Eric J. Gomez, Karl Gerhardt, Justin Judd, Jeffrey J. Tabor
Sentinels of Disease: Engineering Bacteria to Sense and Remember Salmonella Infection in the Mammalian Gut
David T. Riglar, S Jordan Kerns, Jonathan Kotula, Jeffrey C. Way, Pamela A. Silver
New Promoters to Improve Heterologous Protein Production in Penicillium chrysogenum
Fabiola Polli, Roel A.L. Bovenberg, Arnold J.M. Driessen
Metabolic Engineering of Saccharomyces Cerevisiae for Production of Alkanes from Free Fatty Acids
Jee Loon Foo, Adelia Vicanatalia Susanto, Susanna Su Jan Leong, Matthew Wook Chang
Metabolic Engineering of Saccharomyces Cerevisiae for the Overproduction of Short Branched-Chain Fatty Acids
Ai-Qun Yu, Nina Kurniasih Pratomo Juwono, Susanna Su Jan Leong, Matthew Wook Chang
Combinatorial Engineering of Saccharomyces Cerevisiae for Terminal Alkene Production
Binbin Chen, Matthew Wook Chang
Small Molecule–Triggered Cas9 Protein with Reduced Off-Target Cleavage
Kevin M. Davis, David R. Liu
Mammalian Genetic Circuit Construction Utilizing the Q System
Adrian Shimpi, Tara Deans
Large-Scale Determination of Causative Mutations for Enhanced Chemical Tolerance in Evolved Strains of Escherichia coli
Alex Toftgaard Nielsen, Elsayed Tharwat Tolba Mohamed
Introducing Synbis – the Synthetic Biology Information System
Matthieu.A Bultelle, Inaki Sainz de Murieta, Richard I. Kitney
Optimizing heterologous pathways in mammalian cells via competitive splicing
Ryan Richardson, Wade Hicks, Jeffrey C. Way, Pamela A. Silver
Heterologous Expression and Characterization of Hybrid Two-Component Systems from Gut Bacteria
Kristina Daeffler
Implementation of Synthetic Metabolic Pathways for the Production of 2,4-Dihydroxybutyric Acid
Thomas Walther, Clément Auriol, Christopher Topham, Romain Irague, Isabelle Andre, Magali Remaud-Simeon
A General Method for Sensing Small Molecules in Eukaryotes
Justin Feng
Precision Expression Control in Bacteroides Thetaiotaomicron, a Human Commensal Bacterium
Mark Mimee, Timothy K. Lu, Alex C. Tucker
Evaluation of TALE- and Crispr/Cas9-Based Synthetic Transcription Factors for Heterologous Gene Expression in Saccharomyces Cerevisiae
Fabian Machens, Lena Hochrein, Bernd Mueller-Roeber, Katrin Messerschmidt
Engineering Bacteriophages for Specific Bacterial Targeting
Robert J. Citorik, Timothy K. Lu, Mark Mimee
Modular Design of Transcriptional Regulatory Parts By Insulating Operators from Their Cognate Promoters
Chunbo Lou
Logic Gates for Engineering Programmable Interactions in Microbial Consortia
Alex Beliaev, Ryan McClure, Hans C. Bernstein
Hands-Free Control of Heterologous Gene Expression in Batch Cultures
Olivier Borkowski, Drew Endy, Pakpoom Subsoontorn
Engineering Transcriptional Regulator Effector Specificity Using Computational Design and in Vitro Rapid Prototyping: Developing a Vanillin Sensor
Emmanuel L.C. de los Santos, Joseph T. Meyerowitz, Stephen L. Mayo
Construction of Novel Malate Sensing Recombinant Escherichia coli By Engineering of Bacterial Two-Component System
Epathoptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways
Daniel Lachance, Victoria Vernacchio, Matthew Lebovich, Abhijit Shirke, Victor Schultz
Engineering Bacterial Microcompartments to Shield Toxicity during Protein Expression and Purification
Mimi C. Yung, Feliza A. Bourguet, Timothy S. Carpenter
Complex Design, Simple Implementation: The Development of Red Light-Regulated Nuclear Protein Shuttling and Enzyme-Free DNA Assembly
Hannes M. Beyer, Samuel Juillot, Kathrin Herbst, Uwe Straehle, Wilfried Weber, Matias D. Zurbriggen, Patrick Gonschorek, Sophia L. Samodelov, Eberhard Schaefer, Winfried Roemer, Ferenc Nagy, Konrad Mueller
Metabolic Engineering of Ralstonia Eutropha for Biosynthesis of Natural and Unnatural Polyhydroxyalkanoates from Renewable Resources
Crispathbrick: Modular Combinatorial Assembly of Type II-a Crispr Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E. coli
Ö. Duhan Toparlak, Sanjay Guleria, Matthew Lebovich, Jessica T. Stieglitz, Jacob A. Englaender
Development of Modular, Self-Assembling Synthetic Protein Complexes As Screening Platforms for Enhanced Enzyme Discovery
Ian Haydon, Johnnie Hahm, Elizabeth Znameroski, Fang Liu, Tia Heu, Sumati Hasani, Michael Lamsa, Aubrey Jones, William Widner, Paul Harris, Ronald Mullikin, Sarah A., Teter, Janine Lin
Upgrading the Alkane Precursor Pool through Thioesterase Engineering
Marika Ziesack, Brendon Dusel, Jeffrey C. Way, Pamela A. Silver
Bridging the Gap: A Roadmap to Breaking the Biological Design Barrier
Jacob Beal
Optogenetic Regulation of Proteolysis Using an Engineered Light-Switchable Split-Tev Protease
Karl Gerhardt, Jeff Tabor
High-Throughput Picodroplet-Based Analysis of Biosynthetic Libraries
Carlos Bricio, Tom Ellis, Clive Smith, Marian Rehak, Xin Liu, Xin Li
Cell-Free Systems for Synthetic Biology
Michael C. Jewett
Synthetic Biology in a Chip: A Digital Microfluidic Platform for Cell Transformation, Culture and Expression
Philip Gach, Anup K. Singh, Steve C. C. Shih, Nathan J Hillson, Paul D. Adams