GVL Pretreatment for Enzymatic Hydrolysis and Lignin Extraction from Lignocellulosic Biomass

In this study we adapted ball milled biomass as a model substrate in the bench type batch reactors for the pretreatment to observe effects on enzymatic hydrolysis and lignin yield. The experiments were intitally conducted in an oil bath for the conditions of temperature of (60-100 ⁰C), process time of (24- 48 Hrs) & the Aquous GVL concentration was fixed to 80% for all the experiments. Initially, the pinewood was ball milled was different time intervals and 20 Hrs of milling time was screened out to be carried for all the experiments. The lignin yield increased from 3% to 8% when the experiments were performed from 60 C : 24 Hrs to 100 C: 48 Hrs. The analysis of the pretreated biomass lspecifically interms of its composition was carried out. Also the enzymatic digestibility of the fractionated biomass showed a noticeable correlation with the final glucose release . From here it can be conclusively suggested that GVL can be one potential option for the pretreatment of lignocellulosic biorefineries.