4th International Conference on Plant Synthetic Biology, Bioengineering, and Biotechnology
Cr-Sphere: A Toolbox to Study Chlamydomonas Microbiota Interactions
Authors
Flores-Uribe, J. - Presenter, Max Planck Institute for Plant Breeding Research
Duran, P., Max Planck Institute for Plant Breeding Research
Garrido-Oter, R., Max Planck Institute for Plant Breeding Research
Microalgae and bacteria interact in the environment by exchanging diverse nutrients and metabolites. Despite the relevance of these ecological interactions the association between soil microalgae and their microbiota remains understudied. To better understand these relationships, we use the model organism Chlamydomonas reinhardtii.
Co-cultivation of C. reinhardtii with soil samples revealed the recruitment of distinct bacterial communities by the microalgae. These bacterial communities overlap taxonomically with the core root microbiota of land plants, suggesting a set of conserved and possibly ancestral assembly principles. Furthermore, a culture collection of C. reinhardtii associated bacteria (designated Cr-Sphere) was established with representatives for 96% of the most abundant bacteria in the algal microbiota. Analysis of 200 genomes from these bacterial isolates revealed taxonomic and functional overlap between the Cr-sphere and other microbial culture collections isolated from plant roots. The Cr-Sphere can be used as a toolbox to study microalgae-bacteria interactions, their effect into the microalgal fitness, response to nutritional stress, or the establishment and evolution of microbial communities.
Co-cultivation of C. reinhardtii with soil samples revealed the recruitment of distinct bacterial communities by the microalgae. These bacterial communities overlap taxonomically with the core root microbiota of land plants, suggesting a set of conserved and possibly ancestral assembly principles. Furthermore, a culture collection of C. reinhardtii associated bacteria (designated Cr-Sphere) was established with representatives for 96% of the most abundant bacteria in the algal microbiota. Analysis of 200 genomes from these bacterial isolates revealed taxonomic and functional overlap between the Cr-sphere and other microbial culture collections isolated from plant roots. The Cr-Sphere can be used as a toolbox to study microalgae-bacteria interactions, their effect into the microalgal fitness, response to nutritional stress, or the establishment and evolution of microbial communities.