(588aj) Synthesis of Unilammellar Liposomes for Drug Delivery Using Colloidal Packed Bed

Liposome-based drug delivery systems synthesized using colloidal packed bed technology represent a promising advancement in targeted and efficient therapeutic delivery. We highlight the synthesis and application of liposomes through colloidal packed beds, with particular emphasis on their potential for DNA and RNA delivery as well as the treatment of iron deficiency anemia. The colloidal packed bed uses alumina oxide as packing material within an alumina cylindrical tube sintered at 1100 ºC for 30 minutes. Our synthesis process used one-step drying and hydration of lipid films in the packed bed to form desirable small unilamellar liposomes. Additionally, we can obtain low polydisperse liposomes and load cargoes such as iron and DNA. We also aim to achieve higher encapsulation efficiency of iron using anionic lipids such as 1,2-Dipalmitoyl-sn-glycero-3-phosphoglycerol, sodium salt (DPPG), and DNA using the cationic lipid 1,2-Dioleoyl-3-trimethylammonium propane (DOTAP), as well as the helper lipid 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE) and PEGylated lipid (ALC-0159). We aim to adapt our method to accommodate various nucleic acids, including RNA and siRNA, for downstream applications. Synthesized liposomes were measured for size using nanoparticle tracking analysis (NTA) and dynamic light scattering (DLS), while the bilayer lamellarity was confirmed using a Cryo-Transmission Electron Microscope (Cryo-TEM). The encapsulation technique using a colloidal packed bed aims to provide an alternative to existing techniques, such as microfluidics and the impinging jet mixer, which are currently used for the synthesis of liposome-based drug delivery systems.