Microbial-based biomanufacturing relies heavily on fixed nitrogen as an essential substrate for growth and bioprocessing. However, current practices for nitrogen fixation incur significant carbon emissions and require centralized operations, thereby limiting the sustainability and deployability of biotransformation. Here, we present an integrated biomanufacturing framework that combines designer, plasma-based nitrogen fixation with microbial assimilation and conversion for bio-based production. Using the bacteria Escherichia coli and Pseudomonas putida, we demonstrated the feasibility of our framework and further tailored plasma-based fixation to align with microbial metabolic preferences. The resulting nitrogen products exhibited high nutrient quality and high compatibility with diverse carbon sources, thus supporting the metabolism of various microbial chassis. From concept to application, we illustrated the utility of the framework by successfully producing functional biomolecules and upcycling plastic into valuable chemicals. Our work establishes a versatile and sustainable paradigm for microbial nitrogen assimilation and bioproduction, offering broad applications across diverse biomanufacturing contexts.
