2025 AIChE Annual Meeting
(273f) Metabolic Engineering and Biocatalysis of Non-Model Rhodococcus Ruber Chasis Cells
Non-model microorganisms are of great importance due to their special phenotypes such as unique metabolic characteristics, high tolerance against extreme conditions or special features for eznyme expression. Rhodococcus spp. are a group of non-model gram-positive actinomycetes with diverse catabolic activities and strong adapting abilities, enabling their wide applications in whole-cell biocatalysis, lignocellulosic biomass conversion and biodegradation etc. The fundamentals of R. ruber were investigated, such as tolerance mechanism, colony-dimorphism, urea inducible mechanism and non-pigmented regulation. Expanding R. ruber as a universal host for expression of different enzymes was also highlighted. Biocatalysis applications of R. ruber were discussed for the effective bio-production of diverse chiral/valuable products. These studies were supported by the NSFC of China (No. U24A6011) and Beijing Municipal Science & Technology Commission No. Z231100003723015.