The kinetic parameter determination of trans-oligomerization of oligosaccharides in biological systems is critical for understanding carbohydrate metabolism and its implications in various biochemical processes for therapeutic purpose. This study investigates the trans-oligomerization reactions of digestible oligosaccharides to non-digestible oligosaccharide, focusing on the efficiency, initial reaction rates, product yields and inhibition rate under physiological conditions to establish type of reaction mechanism. Utilizing advanced techniques such as absorbance spectrophotometer and kinetic modeling, to assess concentrations of substrate and product of oligosaccharide to glucose, and glucose to non-digestible oligosaccharide, their interactions with enzymatic catalysts in vitro while estimating kinetic parameters based on predetermined reaction mechanisms and measured concentration. Our findings will contribute to a deeper understanding of oligosaccharide reaction kinetic behavior in biological systems, a potential application in glucose management and optimization in carbohydrate transformation.
