Rapid industrialization and population growth have significantly increased global waste pollution, including agricultural residues, plastic waste, and emissions. For example, over 300 million tons of plastic waste are generated annually worldwide. Traditional disposal methods such as landfilling and incineration are economically costly and environmentally harmful, highlighting the need for sustainable alternatives. Green biomanufacturing, leveraging engineered microbes, provides an effective solution by converting waste into valuable products. My research integrates bioprocess optimization, microbial metabolism coordination, and microbial community labor division for efficient waste valorization. Specifically, a SMART surface display platform enabled dynamic regulation of multi-enzyme cascades, greatly improving mixed-sugar conversions. Furthermore, consolidated bioprocess strategies significantly enhanced cellulose-to-butanol conversion, achieving record yields in monocultures. Additionally, a microbial division-of-labor community efficiently converted PET plastics into high-value compounds such as mcl-PHA. These innovations collectively establish a multi-scale microbial platform, advancing biomanufacturing capabilities in sustainable waste management.
