The development of biomaterials that can modulate cellular behavior is critical for advancing tissue engineering and regenerative medicine. Hydrogels, particularly those modified with adhesive peptides, offer promising substrates for cell culture by mimicking the extracellular matrix (ECM). Here, we present the design of a hydrogel system incorporating combinatorial adhesive peptides to regulate cellular adhesion and behavior. By varying the stiffness and peptide presentation, this platform aims to offer precise control over cell-ECM interactions. In contrast to traditional tissue culture dishes, which are pre-coated with matrix proteins to support cell adhesion, this hydrogel system offers an inexpensive, tunable alternative that eliminates the need for matrix pre-coating while maintaining ligand-specific cell attachment. The hydrogel system can be applied across various biological studies, offering control over mechanical properties and biochemical cues, independently, thereby facilitating a versatile substrate for cell culture and tissue engineering applications.
