(182z) Comparative Analysis of Genetic Circuit Behavior across Bacterial Species

Genetic circuit performance is highly dependent on host-specific regulatory and physiological contexts, limiting the transferability of synthetic biology tools across bacterial species. This study investigates the influence of host chassis on the function of inducible genetic circuits by comparing gene expression outputs in Escherichia coli, Pseudomonas putida, and Cupriavidus necator. Circuits incorporating tetracycline- and quorum-sensing-responsive promoters, coupled with ribosome binding sites of varying translational strengths, are evaluated using GFP as a reporter. The design enables systematic assessment of induction dynamics and basal expression levels across hosts, generating a foundational resource to guide the rational selection and adaptation of regulatory systems in emerging and non-model bacterial platforms.