Recently, a binary protein mixture of hemoglobin and bovine serum albumin (BSA) was successfully separated into two highly purified fractions by using internally staged ultrafiltration (ISUF) involving a stack of three identical 100 kDa ultrafiltration (UF) membranes (a) in an Amicon stirred cell. More recently (b), successful separation and purification of IgG from a binary mixture of IgG and BSA containing 1.0 mg/ml IgG and 0.1mg/ml BSA was demonstrated in an Amicon stirred cell using a modified ISUF technique containing two 100 kDa membranes and a 70kDa membrane; here BSA was used as a model for host cell proteins (HCPs). The selectivities achieved in these two systems were as high as 1000-4000+. Individual component recoveries were also quite high. Here we explore separation and purification achievable with similar binary systems in a larger scale device. Results will be presented and performances compared with those from the small cells used earlier. Potential utility of such devices for separation and purification in membrane-based biopharmaceutical separation techniques will be discussed.
References:
(a) Y. Song, L. Feng, S. Basuray, K. K. Sirkar*, S. R. Wickramasinghe, Hemoglobin-BSA Separation and Purification by Internally Staged Ultrafiltration, Separation and Purification Technology, 312, 123363 (2023).
(b) L. Feng, Y. Song, S. Basuray and K.K. Sirkar*, IgG-BSA Separation and Purification by Internally Staged Ultrafiltration, Separation and Purification Technology, 354,129245 (2025).
