At Manus, we have developed a comprehensive BioAlternatives cell engineering and scale-up platform, facilitating the cost-effective and environmentally friendly production of conventionally scarce complex natural products. Our platform utilizes a systems-level approach by integrating chassis design, microbial cell factory engineering, and biomanufacturing at scale. In this presentation, we will share key insights and core principles behind our approach to cell factory engineering using our AI-enhanced BioOptimization Cycle and highlight specific case studies of successfully scaling, manufacturing and commercializing BioAlternatives.