2024 AIChE Annual Meeting
Protein 14-3-3 Expression on Yeast Surface Display
The protein 14-3-3 has seven different isoforms and is known to cause many diseases including neurological diseases, neuropsychiatric diseases, and cancers. 14-3-3 is known to have a weak binding affinity to several other proteins which could explain why it is implicated in a wide variety of disorders. 14-3-3ζ is known to be linked to neurological diseases such as Parkinson’s Disease and Alzheimer’s Disease. 14-3-3η is linked to dementia and Parkinson’s Disease [2]. The goal of this research is to gain a deeper understanding of the 14-3-3 protein and express 14-3-3 using yeast surface display to detect weak protein binding to its target. Yeast surface display is commonly used in protein engineering to express recombinant proteins which are linked to the surface of yeast cells in the extracellular space [1]. Expressing 14-3-3 will lead to insights about how this protein interacts and binds to other proteins in a living organism. Yeast surface display has been used by our lab previously to screen libraries of nanobodies, antibodies, and single chain variable fragments (scFv) against novel binders to a variety of targets. By understanding the avidity or the strength of multiple affinities, more can be learned about 14-3-3's selectivity. With past data supporting yeast surface display to detect weak protein binding interactions, this technique will be employed in a similar way. Overall, 14-3-3 will be expressed on yeast surface display.
References:
[1] Cherf GM, Cochran JR. Applications of Yeast Surface Display for Protein Engineering. Methods Mol Biol. 2015;1319:155-75. doi: 10.1007/978-1-4939-2748-7_8. PMID: 26060074; PMCID: PMC4544684.
[2] Foote M, Zhou Y. 14-3-3 proteins in neurological disorders. Int J Biochem Mol Biol. 2012;3(2):152-64. Epub 2012 May 18. PMID: 22773956; PMCID: PMC3388734.