Layer-By-Layer Nanofilm Coated Hollow Glass Microspheres for Rapid Isolation and Recovery of Salmonella in Resource-Limited Settings

Efficient and rapid detection of salmonella and other bacterial infections is needed to reduce infections in low-income settings. The current detection methods are not viable due to high costs and long timeframes. Conventional approaches require 4 to 5 days for results, and methods such as Surface-Enhanced Raman Scattering (SERS) remain inaccessible due to their complex nature and high expense.

This study provides an alternative bacterial isolation system using self-floating hollow glass microspheres (HGMS) coated with layer-by-layer (LbL) nanostructured polymetric films. Using anti-salmonella antibodies, these microspheres target and rapidly capture salmonella cells. HGMS facilitates bacteria cell isolation within 3 hours, confirming Salmonella presence within 24 hours. This systematic approach reduces detection time while reducing cost, making this a more ideal approach for resource-limited areas.