The Effect of Cellular Microenvironment on the Mechanical Behavior of Cancer Cells

The impact of host environment on metastatic cancer cells have been extensively studied. However, the biophysical and mechanical components of the metastatic cascade is poorly understood. In this study, we investigated the effect of different environments on metastatic cancer cell through conditioned media analysis to understand the mechanical differences between brain and breast cancer cells under varying environmental conditions and how these differences influence their properties. We hypothesized that cancer cells would acquire mechanical properties resembling those of the environment they are exposed to. To test this, we cultured brain cancer cells in a normal breast cancer environment and breast cancer cells in a normal brain cell environment. The cells were subjected to conditioned media for twenty-four hours to assess the mechanical properties of their respective environments. Subsequently, a shear assay method was employed to apply stress to the cells and observe their response and resistance. Data analysis was performed using computer programs such as DaVis and MATLAB. The results were utilized to visualize cell deformation and characterize their mechanical properties. Notably, the cells exhibited responses to the different environments, indicating changes in their mechanical properties when placed in unfamiliar conditions. Surprisingly, even cells of the same type cultured in the same environment exhibited varying responses.

This study sheds light on the dynamic nature of cancer cells and their ability to adapt to different environments. By understanding how mechanical properties are influenced by the host environment, we gain valuable insights into cancer disease and its complex mechanisms. Further research in this area is crucial for advancing our understanding of metastasis and developing innovative diagnostic and therapeutic approaches.