(174aa) Novel IMAC Absorption Size Exclusion Chromatic Matrices for Detection and Isolation of Biomarkers for Early Detection of Diseases from Complex Biological Fluids

This work describes the synthesis of novel absorption chromatographic matrices for the separation and isolation of low molecular weight peptides and small proteins from complex biological fluids. Immobilized metal affinity chromatography (IMAC) was incorporated into these platforms for the binding of targeted biomarkers. Polymers such as m-PEG were incorporated to restrict large proteins from binding and interfering with the targeted peptides. Angiotensin I and II were used as model biomarkers (small molecular weight peptides with 1.3 and 1.015 kDa respectively). A complete separation of both angiotensins was achieved from human serum with two specifically designed ligands, iminodiacetic acid (IDA) and dipicolylamine (DPA), and different metal ions. Similarly, myoglobin was isolated from saliva as a biomarker for acute myocardial infarction.