(127a) An Exploration of the Mechanisms and Kinetics of Nanozymes from a Chemical Engineering Perspective.

Nanozymes are a category of nanomaterials that have inherent properties similar to enzymes. Currently, nanozymes are being used not only for molecular detection but also for environmental protection and cancer diagnosis and therapy. Nanozymes possess exceptional characteristics, combining the benefits of natural enzymes and artificial enzymes. These include remarkable activity and selectivity, adjustable activity levels, remarkable stability, and the ability to be reused. As a result, nanozymes have demonstrated significant potential and extensive applications in various fields such as medicine, agriculture, the environment, food, and pharmaceuticals. The potential uses of nanozymes create a new opportunity for nanomaterials. Nanozymes have enzymatic activities similar to oxidase, peroxidase, superoxide dismutase, and catalase enzymes. Nanozymes possess greater stability compared to natural enzymes and are capable of demonstrating activity across a broad spectrum of pH levels and temperatures. Nanozymes have lower production costs compared to natural enzymes, and they have the advantage of extended storage durations.

An essential inquiry in this domain is around the operational mechanism of these nanozymes. Most of the literature assumes that nanozymes exhibit enzymatic kinetics that are consistent with Michaelis-Menten kinetics. Nevertheless, there may be concerns over the overall applicability of this kinetic approach. Therefore, this study constructs and evaluates various kinetic models that rely on heterogeneous surface reaction processes using a comprehensive fitting algorithm. Ultimately, a comparative evaluation is conducted to analyze the Michaelis-Menten, Langmuir-Hinshelwood, and Eley-Rideal mechanisms and kinetics.