2022 Annual Meeting
(534b) Application of Protocatechuic Acid-Based Deep Eutectic Solvent for Utiliztion of Engineered Biomass
Authors
Xianzhi Meng, University of Tennessee-Knoxville
Yang Tian, Lawrence Berkeley National Laboratory
Linjing Jia, SUNY College of Environmental Science and Forestry
Aymerick Eudes, Joint BioEnergy Institute, Lawrence Berkeley National Laboratory
Kwang Ho Kim, University of British Columbia
Yunqiao Pu, Oak Ridge National Laboratory
Gyu Leem, State University of New York College of Environmental Science and Forestry
Deepak Kumar, SUNY College of Environmental Science and Forestry
Jeong Jae Wie, Inha University
Arthur Ragauskas, University of Tennessee
Chang Geun Yoo, SUNY ESF
To efficiently valorize lignin and achieve a sustainable biorefinery process, genetically engineered sorghum bagasse with altered lignin monomers was pretreated by a novel protocatechuic acid-based deep eutectic solvent (DES). We synthesized the DESs with protocatechuic acid (PCA) and choline chloride (ChCl) and applied this solvent to fractionate sorghum bagasse. The transgenic sorghum bagasse having protocatechuate incooperated lignin was used as a feedstock in this study. Additionally, three-component DESs containing ChCl, PCA and water or ethylene glycol were formed and tested. The fractionated lignin from the black liquor was subjected to hydrothermal treatment to recover protocatechuic acid, and the cellulose-rich pulp was enzymatically hydrolyzed. Structures of isolated lignin before and after hydrothermal treatment were characterized by two-dimensional heteronuclear single quantum (2D HSQC) NMR and gel permeation chromatography, while the liquid products of hydrothermal treatment were analyzed by gas chromatography-mass spectrometry (GC-MS).