(340h) How to Drive a Bio Macromolecule DNA Faster through Nano Pores

The aim of separating DNA fragments in human genome research has stimulated efforts to understand molecular processes underlying electrophoretic separation rate resulting in rapid development of electrophoretic techniques in recent years. We used Gaussian white noise to enhance the mobility of 1kbp DNA band to ⁓ 86 % and 6kbp DNA band to ⁓113 % in comparison to conventional known agarose gel electrophoresis. The experimental setup was similar to conventional agarose gel electrophoresis phenomena under the influence of bias voltage along with the addition of Gaussian noise in the form of voltage pulses. Displacement of the gel bands are tracked from the well bottom to the middle point of a particular band using ImageJ tracking software. The drift velocity of the DNA induced by activated “noise-lubricity”, follows the Arrhenius-Eyring-like escape rate at the high power of the external noise. Whereas, at the low power, the cooperative dynamics of the DNA molecules impart super Arrhenius-like behavior. Gaussian white noise overcomes the pinning barrier of the molecules, highlighting the symmetry breaking for free random motion of macromolecules. The developed model is interpreted in terms of mobility, interaction and dynamics of the DNA molecules in gel electrophoresis setup. Simulation based on Langevin framework predicted a similar enhancement of mobility across the gel interface matrix. The simulation results also pointed on the increasing mobility fluctuations with increasing power of noise which truly resonates with our findings from the experimental results (as shown in graphical plot attached). This work suggests strategies for noise-activated faster DNA fingerprinting and set up the basis for advanced research on resonance-induced super mobility for the isolation of a specific protein fragment from a crowd.