(2bt) Comparative Analysis of Cell-Free Synthesis Systems Based on the Extracts Different Microorganisms.

The fields of synthetic biology are rapidly growing in the disciplines of science, medicine, pharmaceuticals, energy, and chemical industry. Although a variety of genetic tools and methods have been developed for the engineering of diverse organisms, the use of live cells always suffers the intrinsic limitations associated with the presence of physical membranes. In comparison, cell-free protein synthesis system is an open system that is readily accessible to unlimited conditions of protein synthesis. As it does not rely on the growth of cells, cell-free protein synthesis also provides great advantages in the throughput of protein generation. Since Nirenberg and Matthaei first demonstrated that the process of translation can be reproduced in the extract of E. coli [1], cell-free synthesis systems from various organisms have been established, including E. coli, plants, insect, yeast, and mammalian cell lines [2]. This broadens the range of choice for the chassis to produce recombinant proteins depending on their genetic sources. In this study, we constructed cell-free synthesis systems based on different microorganisms such as E. coli, Vibrio natriegens, Corynebacterium glutamicum, and Synechocystis sp., and compared, the key parameters that affect the efficiency and performance of each systems.