(343a) Investigation of Electrochemical Redox Tags for Elastin-like Polymer Sensing

Elastin-Like Polymers (ELP) have been investigated as surface constructs to promote surface interactions or as a stimuli-responsive surface.^1,2 We have previously demonstrated using electrochemical impedance spectroscopy to detect an ELP collapse response.¹ However, EIS measurements are exceptionally sensitive to minute changes in solution or surface conditions, which makes consistent data acquisition difficult.³ In order to create a more robust signal to the same collapse response, we propose using an electrochemical tag coupled with voltametric techniques to recognize the surface response. Differences in electrochemical characteristics of these compounds in gold electrodes versus glassy carbon electrodes will be the focus of this poster.

1. Su, Z., Kim, C. O. and Renner, J. N. Quantification of the effects of hydrophobicity and mass loading on the effective coverage of surface-immobilized elastin-like peptides. Biochem. Eng. J. 168, 107933 (2021). 10.1016/j.bej.2021.107933

2. Morales, M. A., Paiva, W. A., Marvin, L., Balog, E. R. M. and Halpern, J. M. Electrochemical characterization of the stimuli-response of surface-immobilized elastin-like polymers. Soft Matter 15, 9640–9646 (2019). 10.1039/c9sm01681c

3. Bogomolova, A., Komarova, E., Reber, K., Gerasimov, T., Yavuz, O., Bhatt, S. and Aldissi, M. Challenges of Electrochemical Impedance Spectroscopy in Protein Biosensing. Anal. Chem. 81, 3944–3949 (2009). 10.1021/ac9002358 https://pubs.acs.org/doi/10.1021/ac9002358