2020 Virtual AIChE Annual Meeting
(8d) Using a High Throughput Cell Culture Workflow for Process Development and Process Characterization for Therapeutic Proteins
Authors
This workflow begins with the use of ambr15⢠and ambr250⢠(Sartorius) bioreactor systems. Traditionally, bench-scale bioreactor systems (2-L to 5-L) were used for process development and process characterization, but these bioreactors have a longer turnaround time and require more resources for preparation and study execution. Use of disposable ambr⢠bioreactor systems enable scientists to perform 24 to 48 bioreactor runs in parallel, with significantly lower resource need. Sample preparation and sample handling with high throughput bioreactor systems can be challenging. We have implemented an approach for sample preparation using a sample numbering system, TubeWriter, plate centrifuges, Opentron liquid handler and sample tracking using barcodes. This workflow and procedures have been harmonized across all groups in the department.
We will present our approach for establishing this workflow, resource and throughput comparison between traditional bioreactor system and ambr⢠systems, efficiency gain in sample preparation and sample handling using the high throughput technologies. We consider high throughput as a means for continuous improvement to meet our goal of Gene to GMP in 9 months.