(790a) Using microcontact printing to mimic Cardiomyocyte regeneration

Cardiac diseases are the most prominent cause of death worldwide. Cardiomyocyte regeneration is imperative, due to the human body lacking in the ability to sufficiently regenerate cardiomyocytes (CMs). One-way cardiomyocytes can be regenerated is through differentiating human induced pluripotent stem cells(hiPSCs) into cardiomyocytes. In order to attach hiPSCs derived-cardiomyocytes in a manner that would mimic native conditions, various techniques can be used, such as microcontact printing. The microcontact printing technique allows for a specified geometry or pattern to be transferred onto the substrate that will be used for cardiomyocyte seeding. The pattern utilized in this study comes from the rectangular-pillared-array stamp. The preliminary results show that a general cell line of mouse embryonic fibroblasts (MEFs) can be constrained to this pattern.