(621b) Online LC in Support of Data Rich Experimentation

Online Liquid Chromatography analysis is becoming increasingly important for studying the kinetic profiles of chemical reactions, from which valuable mechanistic information can be obtained. Historically, online sample collection was performed via either flow of the reaction medium through a sample loop, followed by injection, or by metered withdrawal of a defined volume flowing via a sampling tube or capillary. Both of these approaches work well for homogeneous solutions, but are not well suited to the investigation of heterogeneous reactions where solids are present or where active precipitation or crystallization is taking place. The recently introduced Mettler Toledo EasySampler 1210 addresses this limitation, allowing for reliable sampling of heterogeneous reactions using a mechanically actuated, 20 µL fixed-pocket based sampling mechanism. However, this tool is limited to the collection of a carousel of 12 samples, limiting the utility for detailed kinetic profiling with frequent, and extending sampling needs, especially when there is poor sample stability of the diluted solution. In this work, we demonstrate expansion of the EasySampler's application range to include direct injection into an LC stack showcasing the EasySampler as a robust sampling solution enabling high data density experimentation.