In spite of the increasingly serious problem of global energy shortage and environmental deterioration, butanol has been developed as a promising renewable energy because butanol has some characteristics better than that of ethanol. Traditional ABE fermentation use strict anaerobic strain to produce butanol. In our research, a new symbiotic strain TSH06 was isolated to produce butanol, which could produce butanol under micro-aerobic condition. The results indicated that ratio of acetone: butanol: ethanol reached 1:8:1, butnaol yield reached 0.31, and butanol concentration reached 19.2g/L, respectively in batch fermentation after optimized technology. Furthermore, butanol fermentation integrated with gas stripping technology was also investigated.