(689e) Dynamic Measurements of Transcription Factors in Response to Cytokine and Fatty Acid Stimulation

Transcription factors (TF) are key effectors of cell signaling pathways that bind specific sites on chromosomal DNA, followed by a sequence of events resulting in activation or repression of nearby genes. TF networks are interconnected so that any one TF can respond to a variety of signals, and many TF levels are altered by a single stimulus. Moreover, the fact that there are ~2000 different TFs in human and that their activities and levels are dynamic, makes parallel TF level profiling studies complex and challenging.

Here, we present parallel, temporal TF levels measurements in different cell types with multiple stimuli by using our previously developed parallel TF measurement assay. Short-term dynamic changes in the levels of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), Signal transducer and activator of transcription 3 (Stat3), cAMP response element-binding protein (CREB), glucocorticoid receptor (GR), and TATA binding protein (TBP), in breast cancer cells and HepG2 cells after TNF-a and palmitic acid exposure were analyzed. Our results illuminate kinetics of TF levels in response to cytokine and fatty acid exposure, also it demonstrates the utility of parallel TF measurement approaches in the analysis of biological phenomena.