(621d) Predictive Screening of Column Pair Combinations for Two-Dimensional Liquid Chromatography

Predictive Screening of Column Pair Combinations for Two-dimensional Liquid Chromatography

Rebecca K. Lindsey,¹ Dwight R. Stoll,² Peter W. Carr,¹ and J. Ilja Siepmann^1,3†

¹Department of Chemistry and Chemical Theory Center, University of Minnesota, Minneapolis, MN 55455-0431
²Department of Chemistry Gustavus Adolphus College, Saint Peter, Minnesota 56082
³Department of Chemical Engineering and Materials Science University of Minnesota, Minneapolis, MN 55455-0431

Two-dimensional liquid chromatography (2DLC) is a powerful separation technique that employs a second column to further separate fractions taken from the first column; when utilized in an on-line fashion, 2DLC can result in much higher total peak capacities than are achievable with a single column. This computational work addresses the significant challenge of selecting an optimal column pair from the N2 possible combinations (order matters in the on-line approach) by presenting a predictive screening method for column pair selection, and provides insight into the defining characteristics of a good column pair.

^†Corresponding Author. E-mail: siepmann@umn.edu