(249c) Hydrodynamic Effects On Cell Aggregation and Cell Attachment of Suspension Cultures in Small-Scale Cell Culture Vessels

Hydrodynamic Effects on Cell Aggregation and Cell Attachment of Suspension Cultures in Small-Scale Cell Culture Vessels

Ru Zang, Tom Hayes and Jin Yin

45 New York Ave, Genzyme, a Sanofi Company, Framingham, MA 01701

Abstract:

Small-scale vessels for suspension cultures such as shake flasks, spinners and wave bags have been widely used in cell culture processes. We have observed significant cell aggregation and cell attachment (CACA) issues when growing some cell lines in these vessels. In order to resolve CACA issues, different types of small scale cell culture vessels have been applied in this investigation and it was found that CACA was related to hydrodynamics in these vessels. Furthermore, CFD (Computational Fluid Dynamics) analysis of hydrodynamics was conducted to support the understanding of mechanism of CACA in vessels with different mixing patterns. This presentation will share some of our experience and understanding on CACA for suspension culture in different types of vessels. Instead of using anti-clumping agents, a potential approach to reduce CACA by controlling culture hydrodynamics will be discussed as well.