(95f) Control of Lateral Interactions of Surface-Immobilized Antibodies Via Substrate NANO-Patterning

Lateral interaction between immobilized Ab on the surface of immunosensor is important factor that determines biosensor's performance. Such interactions hider the surface diffusion of Ab in liquid solution onto the host surface and increase the degree of non-specific Ab immobilization onto the host surface. We propose to control lateral interactions via topological modification of the host surface.

To investigate the effect of the topography of nano-patterned surface on Ab immobilization and their lateral interaction, nano-porous substrates were prepared by electrochemical anodization of aluminum. The effect of dimension of nano-porous structure on the amount of immobilized Ab was investigated by measuring the fluorescence intensity of FITC-Ab immobilized immunosensor based on nano-porous substrate. Effect of the nano-patterns on the sensitivity of biosensor was tested for detection of Staphylococcus enterotoxin B in complex matrix. Our data show an improved sensitivity and selectivity of developed nanosensor.