2008 Annual Meeting

(613d) Enzymic Thin Film Coatings for Bioactive Materials

Authors

Songtao Wu - Presenter, University of Minnesota
Xiaodong Tong, University of Minnesota
Archana Trivedi, University of Akron
Hongfei Jia, Toyota Motor Engineering and Manufacturing North America, Inc.
Minjuan Zhang, Toyota Motor Engineering and Manufacturing North America, Inc.
Masahiko Ishii, TOYOTA MOTOR CORPORATION
Ping Wang, University of Minnesota
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for reparation of bioactive materials through a spin coating process. Specifically,we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 ug/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.