(494c) A Novel Glucose Biosensor Based on Electrochemically Synthesized Chitosan- Conductive Poly(N-Methylaniline) Composite

In general, the composites obtained by incorporating a rigid conducting polymer (such as PANI) into a flexible matrix (such as chitosan) can combine the good processability of the matrix and the electrical conductivity of the conductive polymer. Copolymerization of polyaniline (PANI) with chitosan can produce nanocomposites that enhance the conductivity of the film. In recent years, polyaniline is becoming a promising material for potentiometic biosensors.

In our work, poly(N-methylaniline)/Chitosan(PNMANI/Ch) composite was electrochemically synthesized in H2SO4 acid medium. The PNMANI/Ch composite was characterized by FTIR and Uv-Vis spectroscopy. The morphological properties of the composite were investigated using SEM analysis. For biosensor applications, glucose oxidase (GOX) was immobilized on the surface of this composite by the physical adsorption method. A sensitive and selective biosensor for glucose was developed by electrochemical oxidation of H2O2. Amperometric detection of glucose was carried out by using the enzyme immobilized PNMANI/Ch composite as the working electrode and calomel electrode as the reference electrode. The Michaelis-Menten constant was calculated as 54.141 mM. Optimization of the biosensor construction as well as effects of experimental variables on the amperometric response of the sensor are discussed.