(333a) Dynamical Analysis of Silver-Staining Gel for 2DE

The time courses of 2DE gel images were analyzed dynamically by our new system, which consist of the intermittent scanner system and the software for the dynamic analysis of the intermittent gel images. The system can recognize protein spots more elaborately compared with the conventional image analyzing system using a single gel image because our system can recognize the overlapped protein spots by use of sequential gel images that were scanned intermittently in the color developing reaction.

Our software can adjust the gel positions on the sequential images by recognition of artificial color points that were inserted on the 2DE gel just before developing process. Furthermore, the time course of each spot image could be displayed and the detailed information were obtained in addition to the spot volume and/or location. Our noble method can analyze 2DE-gel without the harmful influence of the addition time of stopper on the measurement result, and we can detect the difference of the spot of the cancer and non-cancer tissue protein more precisely than those with the normal approach with the image of single developing time.