(130a) Integration Of Microfluidic Combinatorial Chemistry Chips With Photonic Crystal Biosensors

The benefit of combinatorial chemistry stems from doing a large array of reactions using less time and reagent per reaction, which naturally lead to a possible solution using microfluidic chips to perform precisely metered reactions in densely packed arrays. We present a microfluidic well plate chip that can perform a matrix of reactions by crossing reagent streams with a minimum of crosstalk and integrated to a photonic crystal biosensor. The volumes of the individual wells are on the order of fractions of nanoliters, and while this could interfere with an optical readout of the components of the reactors, we manage to sidestep this by incorporating a photonic crystal biosensor that focuses on individual binding events of the fluidic chip. The microfluidic chip is then tested using a proof-of-principle reaction matrix of an antibody/protein array.