2006 AIChE Annual Meeting

(447l) Potential Environmental Application of Self-Assembled Protein Hydrogel Based on Elastin-like Protein Sequences

Authors

Lao, U. L. - Presenter, University of California
Hehn, P., University of California
Jenikova, G., University of California
Mulchandani, A., University of California, Riverside
Chen, W., University of California
Recombinant DNA methods have enabled the creation of protein-based block copolymers with programmable sequences, desired properties and predictable three-dimensional structures. These advantages over conventional polymer counterparts facilitate the utility of this new class of biomaterials in a wide range of applications.

In this project, we exploit the potential environmental application of protein-based block copolymers based on elastin-like protein (ELP) sequences. Triblock copolymers containing charged and hydrophobic segments were synthesized. Chain lengths of each segment were manipulated in order to maintain a gelation point below room-temeperature. Polyhistidine sequences were successfully incoporated into the hydrophilic segment without disruption of the self-assembled hydrogel formation. The microscopic structure was investigated by Laser Confocal Microscopy to evaluate the robustness of gel formation under different conditions.

The metal binding capability and capacity of resulting hydrogel was studied to demonstrate the functionality of polyhistidine and potential application of this genetically engineered hydrogel as a permeable barrier material for underground water treatment. Reversibility of metal binding was demonstrated, indicating the cost-effectiveness of this hydrogel barrier system.