2006 AIChE Annual Meeting
(439b) Isolation, Amplification and Detection of Viral RNA
Authors
Kristina Prachanronarong - Presenter, Brown University
Matthew B. Kerby, Brown University
Tiffany Yizar, Brown University
Anubhav Tripathi, Brown University
Andrew W. Artenstein, Memorial Hospital of RI
Steve M. Opal, Memorial Hospital of RI
Sarah Freeman, Brown University
The poster presents a study on isolation of target sequences, quantifying binding efficiency, and calculations of minimum viral load density needed in the samples of synthetic, non-virulent vRNA sequences for human hemagglutinin (H) and neuraminidase (N) genes. DNA primer/promoters are created to capturing the vRNA by linking to beads for purification. Purified vRNA is converted to cDNA and amplified in a continuous flow reaction using a T7-polymerase. The rates of RNA synthesis, durability, amplification efficiency are evaluated. The synthesized sequences are evaluated with specifically designed molecular beacon sequences identifying a known human influenza subtypes H1N1. The hybridization specificity, reaction time and signal to noise ratios are evaluated.