2005 Annual Meeting
(291o) Single-Walled Carbon Nanotube Detection of Ion-Mediated DNA Polymorphism
Authors
Daniel A. Heller - Presenter, Massachusetts Institute of Technology
Michael S. Strano - Presenter, Massachusetts Institute of Technology
Esther S. Jeng - Presenter, University of Illinois at Urbana-Champaign
Tsun-Kwan Yeung - Presenter, University of Illinois at Urbana-Champaign
Brittany M. Martinez - Presenter, University of Illinois at Urbana-Champaign
Anthonie E. Moll - Presenter, University of Illinois at Urbana-Champaign
Joseph B. Gastala - Presenter, University of Illinois, Urbana-Champaign
The transition of DNA secondary structure from the B to Z conformation was detected via modulation of the intrinsic near-infrared fluorescence of single-walled carbon nanotubes. A reversible shift in emission energy of DNA-encapsulated nanotubes was found to closely match the thermodynamics of the transition of unbound DNA. A 30-mer oligonucleotide on the nanotube surface behaves as if it were shortened by 1/6 due to the propagation length of the B-Z transition. The system motivates new near-infrared biosensors capable of operation in strongly scattering or absorbing media, and we demonstrate the optical detection of the B-Z transition from localized complexes within live mammalian cells.